Overview

  • Product name
  • Description
    Rabbit polyclonal to Ephrin A1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Ephrin A1 aa 83-115. The exact sequence is proprietary.
    Database link: P20827

  • Positive control
    • MCF7 cell lysate; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab199697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 24 kDa.
ICC/IF 1/100.

Target

  • Function
    Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cones.
  • Tissue specificity
    Brain. Down-regulated in primary glioma tissues compared to the normal tissues. The soluble monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells (at protein level).
  • Sequence similarities
    Belongs to the ephrin family.
  • Post-translational
    modifications
    Undergo proteolysis by a metalloprotease to give rise to a soluble monomeric form.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B61 antibody
    • ECKLG antibody
    • EFL 1 antibody
    • EFL1 antibody
    • EFNA 1 antibody
    • Efna1 antibody
    • EFNA1_HUMAN antibody
    • EPH related receptor tyrosine kinase ligand 1 antibody
    • EPH-related receptor tyrosine kinase ligand 1 antibody
    • Ephrin-A1 antibody
    • Ephrin-A1, secreted form antibody
    • EphrinA1 antibody
    • EPLG 1 antibody
    • EPLG1 antibody
    • Immediate early response protein B61 antibody
    • LERK 1 antibody
    • LERK-1 antibody
    • LERK1 antibody
    • Ligand of eph related kinase 1 antibody
    • OTTHUMP00000033242 antibody
    • OTTHUMP00000033271 antibody
    • secreted form antibody
    • TNF alpha-induced protein 4 antibody
    • TNFAIP 4 antibody
    • TNFAIP4 antibody
    • Tumor necrosis factor alpha induced protein 4 antibody
    • Tumor necrosis factor alpha-induced protein 4 antibody
    see all

Images

  • Immunofluorescent analysis of HeLa cells labeling Ephrin A1 with ab199697 at 1/100 dilution. Right panel shows the negative control.

  • Anti-Ephrin A1 antibody (ab199697) at 1/1000 dilution + MCF7 cell lysate at 35 µg

    Secondary
    Goat anti-rabbit IgG H&L(HRP) at 1/5000 dilution

    Predicted band size: 24 kDa

References

This product has been referenced in:
  • Chu M & Zhang C Inhibition of angiogenesis by leflunomide via targeting the soluble ephrin-A1/EphA2 system in bladder cancer. Sci Rep 8:1539 (2018). Read more (PubMed: 29367676) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab199697.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
For licensing inquiries, please contact partnerships@abcam.com

Sign up