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RecombinantRabMAb

Recombinant Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)

  • Datasheet
  • SDS
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Western blot - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)
  • OI-RD Scanning - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)
  • Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1785(2)] to Ephrin A1
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Ephrin A1 antibody [EPR1785(2)]
    See all Ephrin A1 primary antibodies
  • Description

    Rabbit monoclonal [EPR1785(2)] to Ephrin A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Ephrin A1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • HUVEC (treated with TNF alpha), HUVEC (untreated) and MCF7 cell lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.59 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR1785(2)
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Ephrins
    • Ephrins
    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Growth Cone
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Tyrosine Kinase Receptors

Associated products

  • Alternative Versions

    • Anti-Ephrin A1 antibody [EPR1785(2)] - BSA and Azide free (ab248056)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • MCF7 membrane extract lysate (ab29539)
  • Recombinant Protein

    • Recombinant Human Ephrin A1 protein (Fc Chimera) (ab181919)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cones.
    • Tissue specificity

      Brain. Down-regulated in primary glioma tissues compared to the normal tissues. The soluble monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells (at protein level).
    • Sequence similarities

      Belongs to the ephrin family.
    • Post-translational
      modifications

      Undergo proteolysis by a metalloprotease to give rise to a soluble monomeric form.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession P20827 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1942 Human
      • Omim: 191164 Human
      • SwissProt: P20827 Human
      • Unigene: 516664 Human
      • Alternative names

        • B61 antibody
        • ECKLG antibody
        • EFL 1 antibody
        • EFL1 antibody
        • EFNA 1 antibody
        • Efna1 antibody
        • EFNA1_HUMAN antibody
        • EPH related receptor tyrosine kinase ligand 1 antibody
        • EPH-related receptor tyrosine kinase ligand 1 antibody
        • Ephrin-A1 antibody
        • Ephrin-A1, secreted form antibody
        • EphrinA1 antibody
        • EPLG 1 antibody
        • EPLG1 antibody
        • Immediate early response protein B61 antibody
        • LERK 1 antibody
        • LERK-1 antibody
        • LERK1 antibody
        • Ligand of eph related kinase 1 antibody
        • OTTHUMP00000033242 antibody
        • OTTHUMP00000033271 antibody
        • secreted form antibody
        • TNF alpha-induced protein 4 antibody
        • TNFAIP 4 antibody
        • TNFAIP4 antibody
        • Tumor necrosis factor alpha induced protein 4 antibody
        • Tumor necrosis factor alpha-induced protein 4 antibody
        see all

      Images

      • Western blot - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)
        Western blot - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)
        All lanes : Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911) at 1/1000 dilution

        Lane 1 : HUVEC cell lysate, treated with TNF alpha
        Lane 2 : HUVEC cell lysate
        Lane 3 : MCF7 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

        Predicted band size: 24 kDa
        Observed band size: 24 kDa

      • OI-RD Scanning - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)
        OI-RD Scanning - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)
        Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab124911? Please let us know so that we can cite the reference in this datasheet.

      ab124911 has been referenced in 1 publication.

      • Wang Y  et al. HIC1 and miR-23~27~24 clusters form a double-negative feedback loop in breast cancer. Cell Death Differ 24:421-432 (2017). PubMed: 28009350

      Customer reviews and Q&As

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      Please use the links above to contact us or submit feedback about this product.

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