Product nameAnti-Ephrin A1 antibody [EPR1785(2)]
See all Ephrin A1 primary antibodies
DescriptionRabbit monoclonal [EPR1785(2)] to Ephrin A1
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human Ephrin A1 aa 1-100 (N terminal). The exact sequence is proprietary.
- HUVEC (treated with TNF alpha), HUVEC (untreated) and MCF7 cell lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.59 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).|
FunctionPlays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cones.
Tissue specificityBrain. Down-regulated in primary glioma tissues compared to the normal tissues. The soluble monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells (at protein level).
Sequence similaritiesBelongs to the ephrin family.
modificationsUndergo proteolysis by a metalloprotease to give rise to a soluble monomeric form.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- B61 antibody
- ECKLG antibody
- EFL 1 antibody
All lanes : Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911) at 1/1000 dilution
Lane 1 : HUVEC cell lysate, treated with TNF alpha
Lane 2 : HUVEC cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
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