Recombinant
Recombinant Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)
- Datasheet
- References (1)
- Protocols
Overview
-
Product name
Anti-Ephrin A1 antibody [EPR1785(2)]
See all Ephrin A1 primary antibodies -
Description
Rabbit monoclonal [EPR1785(2)] to Ephrin A1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Ephrin A1 aa 1-100 (N terminal). The exact sequence is proprietary.
-
Positive control
- HUVEC (treated with TNF alpha), HUVEC (untreated) and MCF7 cell lysates.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.59 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1785(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
Target
-
Function
Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cones. -
Tissue specificity
Brain. Down-regulated in primary glioma tissues compared to the normal tissues. The soluble monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells (at protein level). -
Sequence similarities
Belongs to the ephrin family. -
Post-translational
modificationsUndergo proteolysis by a metalloprotease to give rise to a soluble monomeric form. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 1942 Human
- Omim: 191164 Human
- SwissProt: P20827 Human
- Unigene: 516664 Human
-
Alternative names
- B61 antibody
- ECKLG antibody
- EFL 1 antibody
see all
Images
-
![Western blot - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)](https://www.abcam.com/ps/products/124/ab124911/Images/ab124911-138902-anti-ephrin-a1-antibody-epr17852-western-blot.jpg)
Western blot - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)All lanes : Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911) at 1/1000 dilution
Lane 1 : HUVEC cell lysate, treated with TNF alpha
Lane 2 : HUVEC cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa -
![Other - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)](https://www.abcam.com/ps/products/124/ab124911/Images/ab124911-138901-anti-ephrin-a1-antibody-epr17852.jpg)
Other - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)
Protocols
Datasheets and documents
References
This product has been referenced in:
- Wang Y et al. HIC1 and miR-23~27~24 clusters form a double-negative feedback loop in breast cancer. Cell Death Differ 24:421-432 (2017). Read more (PubMed: 28009350) »
