Recombinant
Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-Ephrin A1 antibody [EPR1785(2)]
See all Ephrin A1 primary antibodies -
DescriptionRabbit monoclonal [EPR1785(2)] to Ephrin A1
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivityReacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Ephrin A1 aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- HUVEC (treated with TNF alpha), HUVEC (untreated) and MCF7 cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Dissociation constant (KD)KD = 1.59 x 10 -10 M Learn more about KD
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR1785(2)
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab124911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
Target
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FunctionPlays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cones.
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Tissue specificityBrain. Down-regulated in primary glioma tissues compared to the normal tissues. The soluble monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells (at protein level).
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Sequence similaritiesBelongs to the ephrin family.
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Post-translational
modificationsUndergo proteolysis by a metalloprotease to give rise to a soluble monomeric form. -
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 1942 Human
- Omim: 191164 Human
- SwissProt: P20827 Human
- Unigene: 516664 Human
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Alternative names
- B61 antibody
- ECKLG antibody
- EFL 1 antibody
see all
Images
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![Western blot - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)](https://www.abcam.com/ps/products/124/ab124911/Images/Ephrin-A1-Primary-antibodies-ab124911-1.jpg)
Western blot - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)All lanes : Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911) at 1/1000 dilution
Lane 1 : HUVEC cell lysate, treated with TNF alpha
Lane 2 : HUVEC cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa -
![Other - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)](https://www.abcam.com/ps/products/124/ab124911/Images/Ephrin-A1-Primary-antibodies-ab124911-2.jpg)
Other - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)
Protocols
References
This product has been referenced in:
- Wang Y et al. HIC1 and miR-23~27~24 clusters form a double-negative feedback loop in breast cancer. Cell Death Differ 24:421-432 (2017). Read more (PubMed: 28009350) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"