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Mouse monoclonal [OTI3E3] to Ephrin A2
Recombinant full length protein corresponding to Human Ephrin A2 aa 1-213. Produced in HEK293T cells (NP_001396).
MAPAQRPLLPLLLLLLPLPPPPFARAEDAARANSDRYAVYWNRSNPRFHA GAGDDGGGYTVEVSINDYLDIYCPHYGAPLPPAERMEHYVLYMVNGEGHA SCDHRQRGFKRWECNRPAAPGGPLKFSEKFQLFTPFSLGFEFRPGHEYYY ISATPPNAVDRPCLRLKVYVRPTNETLYEAPEPIFTSNNSCSSPGGCRLF LSTIPVLWTLLGS
WB: HEK293T cells transfected pCMV6-ENTRY Ephrin A2 cDNA.
IHC-P: Human endometrium adenocarcinoma tissue.
ICC/IF:COS7 cells transiently transfected by pCMV6-ENTRY Ephrin A2.
Flow: Jurkat cells.
The clone number has been updated from 3E3 to OTI3E3, both clone numbers name the same clone.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituents: 1% BSA, 50% Glycerol, PBS
Concentration information loading...
Purified from cell culture supernatant by affinity chromatography
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Predicted molecular weight: 24 kDa.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Belongs to the ephrin family.
Information by UniProt
EFNA 2 antibody
Western blot - Anti-Ephrin A2 antibody [OTI3E3] (ab123877)
All lanes : Anti-Ephrin A2 antibody [OTI3E3] (ab123877) at 1/2000 dilution Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY Ephrin A2 cDNA Lysates/proteins at 5 µg per lane. Predicted band size: 24 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin A2 antibody [OTI3E3] (ab123877)
Paraffin-embedded human endometrium tissue stained for Ephrin A2 using ab123877 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-Ephrin A2 antibody [OTI3E3] (ab123877)
COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY Ephrin A2, stained for Epherin A2 (Green) using ab123877 at 1/100 dilution in ICC/IF.
Flow Cytometry - Anti-Ephrin A2 antibody [OTI3E3] (ab123877)
ab123877 at 1/100 dilution staining Ephrin-A2 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"