Overview

  • Product name

  • Description

    Rabbit polyclonal to Ephrin A3
  • Host species

    Rabbit
  • Specificity

    ab64814 detects endogenous levels of total Ephrin A3 protein.
  • Tested applications

    Suitable for: WB, ELISA, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthetic peptide derived from the C-terminal of human Ephrin A3.

  • Positive control

    • Extracts from HepG2 and Jurkat cells. IF/ICC: HeLa cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab64814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 26 kDa).
ELISA 1/10000.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use a concentration of 5 mg/ml.

Target

  • Tissue specificity

    Expressed in brain, skeletal muscle, spleen, thymus, prostate, testis, ovary, small intestine, and peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the ephrin family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • EFL 2 antibody
    • EFL-2 antibody
    • EFL2 antibody
    • EFN A3 antibody
    • EFNA 3 antibody
    • Efna3 antibody
    • EFNA3_HUMAN antibody
    • EHK1 L antibody
    • EHK1 ligand antibody
    • EHK1-L antibody
    • EHK1L antibody
    • EPH related receptor tyrosine kinase ligand 3 antibody
    • EPH-related receptor tyrosine kinase ligand 3 antibody
    • Ephrin-A3 antibody
    • EphrinA3 antibody
    • EPLG 3 antibody
    • EPLG3 antibody
    • LERK 3 antibody
    • LERK-3 antibody
    • LERK3 antibody
    • Ligand of eph related kinase 3 antibody
    see all

Images

  • ICC/IF image of ab64814 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64814, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Ephrin A3 antibody (ab64814) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from Jurkat cells
    Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 26 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?

References

ab64814 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1mMEDTA
Permeabilization
No
Specification
Spleen
Blocking step
(agent) for 5 minute(s) · Concentration: 0.5% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 12 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Permeabilization
Yes - 0.1% triton X100
Specification
HEK293
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 31 2017

Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Mouse Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 29 2014

Application
Flow Cytometry
Sample
Human Cell (Jurkat cell line)
Specification
Jurkat cell line
Permeabilization
No

Abcam user community

Verified customer

Submitted Jul 22 2011

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