Product nameAnti-Ephrin A3 antibody
See all Ephrin A3 primary antibodies
DescriptionRabbit polyclonal to Ephrin A3
Specificityab64814 detects endogenous levels of total Ephrin A3 protein.
Tested applicationsSuitable for: WB, ELISA, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
A synthetic peptide derived from the C-terminal of human Ephrin A3.
- Extracts from HepG2 and Jurkat cells. IF/ICC: HeLa cell line.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab64814 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab64814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 26 kDa).|
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 5 mg/ml.|
Tissue specificityExpressed in brain, skeletal muscle, spleen, thymus, prostate, testis, ovary, small intestine, and peripheral blood leukocytes.
Sequence similaritiesBelongs to the ephrin family.
Cellular localizationCell membrane.
- Information by UniProt
- EFL 2 antibody
- EFL-2 antibody
- EFL2 antibody
ICC/IF image of ab64814 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64814, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Ephrin A3 antibody (ab64814) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells
Lane 2 : Extracts from Jurkat cells
Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 26 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
ab64814 has not yet been referenced specifically in any publications.