Key features and details
- Rabbit polyclonal to Ephrin A3
- Suitable for: WB, ELISA, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Ephrin A3 antibody
See all Ephrin A3 primary antibodies
DescriptionRabbit polyclonal to Ephrin A3
Specificityab64814 detects endogenous levels of total Ephrin A3 protein.
Tested applicationsSuitable for: WB, ELISA, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
A synthetic peptide derived from the C-terminal of human Ephrin A3.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab64814 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab64814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 26 kDa).|
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Tissue specificityExpressed in brain, skeletal muscle, spleen, thymus, prostate, testis, ovary, small intestine, and peripheral blood leukocytes.
Sequence similaritiesBelongs to the ephrin family.
Cellular localizationCell membrane.
- Information by UniProt
- EFL 2 antibody
- EFL-2 antibody
- EFL2 antibody
All lanes : Anti-Ephrin A3 antibody (ab64814) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells
Lane 2 : Extracts from Jurkat cells
Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 26 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
ab64814 has not yet been referenced specifically in any publications.