Key features and details
- Rabbit polyclonal to Ephrin A4
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Ephrin A4 antibody
DescriptionRabbit polyclonal to Ephrin A4
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse
Recombinant full length protein corresponding to Mouse Ephrin A4 aa 1-206. Expressed in E. coli. N-terminal His-tag and GST-tag.
MRLLPLLRTVLWAALLGSRLPGCSSLRHPIYWNSSNPRLLRGDAVVELGF NDYLDIFCPHYESPGPPEGPETFALYMVDWSGYEACTAEGANAFQRWNCS MPFAPFSPVRFSEKIQRYTPFPLGFEFLPGETYYYISVPTPESPGRCLRL QVSVCCKESGSSHESAHPVGSPGESGTSGWRGGHAPSPLCLLLLLLLPIL RLLRVL
Database link: O08542
- WB: Recombinant mouse Ephrin A4 protein. Mouse spleen, ovary and thymus tissue lysate. IHC-P: Mouse liver and kidney tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 23 kDa.|
FunctionMay play a role in the interaction between activated B lymphocytes and dendritic cells in tonsils.
Tissue specificityExpressed in the adult spleen, lymph node, prostate, ovary, small intestine, and colon, and in fetal heart, lung, liver and kidney. Also detected in hematopoietic cell lines.
Sequence similaritiesBelongs to the ephrin family.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- EFL 4 antibody
- EFL4 antibody
- EFN A4 antibody
All lanes : Anti-Ephrin A4 antibody (ab232711) at 3 µg/ml
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse ovary tissue lysate
Lane 3 : Mouse thymus tissue lysate
All lanes : HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 23 kDa
Formalin-fixed, paraffin-embedded mouse kidney tissue stained for Ephrin A4 with ab232711 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded mouse liver tissue stained for Ephrin A4 with ab232711 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Anti-Ephrin A4 antibody (ab232711) at 3 µg/ml + Recombinant mouse Ephrin A4 protein
Predicted band size: 23 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232711 has not yet been referenced specifically in any publications.