Product nameAnti-Ephrin B (phospho Y317) antibody
See all Ephrin B primary antibodies
DescriptionRabbit polyclonal to Ephrin B (phospho Y317)
SpecificityThis antibody is specific to Dak Ephrin B protein phosphorylated at Tyr317.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human, Xenopus laevis
Phosphopeptide corresponding to amino acid residues surrounding the phospho Tyr317 of Chicken EphrinB.
- Rat testes lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab33473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Note: The immunolabeling of the Ephrin B band is blocked by lambda phosphatase treatment.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceEphrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase.
Cellular localizationMembrane; single pass type I membrane protein.
- CEK5 ligand antibody
- EFNB1 antibody
- ELK L antibody
All lanes : Anti-Ephrin B (phospho Y317) antibody (ab33473) at 1/1000 dilution
Lane 1 : Rat testes lysate treated with this Ephrin B antibody phosphorylated at Tyr317 (control).
Lane 2 : Rat testes lysate incubated in lambda phosphatase (1200 unit for 30 min) before being exposed to this Ephrin B antibody phosphorylated at Tyr317.
Lysates/proteins at 50 µg per lane.
Predicted band size: 37 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
ab33473 has not yet been referenced specifically in any publications.