Key features and details
- Rabbit polyclonal to Ephrin B (phospho Y331)
- Suitable for: WB
- Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis
- Isotype: IgG
Product nameAnti-Ephrin B (phospho Y331) antibody
See all Ephrin B primary antibodies
DescriptionRabbit polyclonal to Ephrin B (phospho Y331)
Specificityab32586 detects Phospho-EphrinB (Tyr331).
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human, Xenopus laevis
Synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphorylated Tyrosine 331 of chicken Ephrin B
- Rat testes tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab32586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Detects a band of approximately 46 kDa (predicted molecular weight: 38 kDa).|
RelevanceEphrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase.
Cellular localizationMembrane; single pass type I membrane protein.
- CEK5 ligand antibody
- EFNB1 antibody
- ELK L antibody
All lanes : Anti-Ephrin B (phospho Y331) antibody (ab32586)
Lane 1 : rat testes lysate
Lane 2 : rat testes lysate with previous incubation with 1200 units of lambda-phosphatase for 30min
Predicted band size: 38 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
ab32586 has not yet been referenced specifically in any publications.