Overview

  • Product name
  • Description
    Rabbit polyclonal to Ephrin B2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Ephrin B2 aa 328-332 conjugated to keyhole limpet haemocyanin.
    Sequence:

    N-I-Y-Y-K


    Database link: P52799

  • Positive control
    • HeLa cells; HT29 cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab131536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 36 kDa.
ICC/IF 1/100 - 1/200.
Flow Cyt 1/100.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
    (Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.
  • Tissue specificity
    Lung and kidney.
  • Sequence similarities
    Belongs to the ephrin family.
    Contains 1 ephrin RBD (ephrin receptor-binding) domain.
  • Post-translational
    modifications
    Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EFN B2 antibody
    • EFNB 2 antibody
    • Efnb2 antibody
    • EFNB2_HUMAN antibody
    • Eph related receptor tyrosine kinase ligand 5 antibody
    • EPH-related receptor tyrosine kinase ligand 5 antibody
    • ephrin B2 antibody
    • Ephrin-B2 antibody
    • EphrinB2 antibody
    • EPLG 5 antibody
    • EPLG5 antibody
    • Htk L antibody
    • HTK ligand antibody
    • HTK-L antibody
    • HTKL antibody
    • LERK 5 antibody
    • LERK-5 antibody
    • LERK5 antibody
    • Ligand of eph related kinase 5 antibody
    • MGC126226 antibody
    • MGC126227 antibody
    • MGC126228 antibody
    • OTTMUSP00000024973 antibody
    see all

Images

  • ab131536 staining Ephrin B2 in formalin-fixed, paraffin embedded rat C6 cells. Samples were incubated with primary antibody at 1/75 dilution. A Goat anti-rabbit IgG H&L (1/50) was used as the secondary antibody. 

  • Immunohistochemical analysis of rat kidney tissue sections at 1/100 dilution. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. A HRP-conjugated goat anti-rabbit antibody was used as the secondary.

  • Immunohistochemical analysis of mouse kidney tissue sections at 1/100. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

  • Anti-Ephrin B2 antibody (ab131536) at 1/500 dilution + HT29 cell extract

    Predicted band size: 36 kDa

  • Immunohistochemical analysis of Human renal clear cell carcinoma tissue sections labeling Ephrin B2 with ab131536 at 1/200. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. the tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. A HRP-conjugated Goat Anti-Rabbit antibody was used as the secondary.

  • ab131536 staining Ephrin B2 in formalin-fixed, paraffin embedded HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1/75 dilution. A Goat anti-rabbit IgG H&L (1/50 dilution) was used as the secondary antibody.

  • Immunofluorescence analysis of methanol-fixed HeLa cells labelling Ephrin B2 with ab131536 at 1/100 dilution.
  • Immunohistochemical analysis of Human meningeal carcinomatosis (MS) tissue sections labeling Ephrin B2 with ab131536 at 1/200. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. the tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. A HRP-conjugated Goat Anti-Rabbit antibody was used as the secondary.

  • All lanes : Anti-Ephrin B2 antibody (ab131536) at 1/1000 dilution

    Lane 1 : Rat spleen tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Peptide blocked mouse liver tissue lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 36 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/Immunofluorescence analysis of TM 3 cells labelling Ephrin B2 with ab131536 at a 1/75 dilution. A Cy3-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a 1/50 dilution.

References

This product has been referenced in:
  • Li X  et al. Atorvastatin regulates pericardial patch healing via the microRNA140-ADAM10-ephrinB2 pathway. Am J Transl Res 10:4054-4064 (2018). Read more (PubMed: 30662650) »
  • Planagumà J  et al. Ephrin-B2 prevents N-methyl-D-aspartate receptor antibody effects on memory and neuroplasticity. Ann Neurol 80:388-400 (2016). Read more (PubMed: 27399303) »
See all 6 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 100X Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% tween 20
Specification
Liver
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde

Mr. David Ivancic

Verified customer

Submitted Jun 18 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (kidney)
Permeabilization
No
Specification
kidney
Blocking step
Serum as blocking agent for 25 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted May 04 2017

Application
Flow Cytometry
Sample
Mouse Cell (Human HEK293 transfected with mouse EFNB2)
Specification
Human HEK293 transfected with mouse EFNB2
Preparation
Cell harvesting/tissue preparation method: Trypsin
Sample buffer: PBS 0.2%FBS
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.2% TritonX-100 in PBS for 15 minutes
Gating Strategy
Single cell

Mr. David Sanchez Martin

Verified customer

Submitted Feb 12 2013

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