Product nameAnti-Ephrin B2 antibody
See all Ephrin B2 primary antibodies
DescriptionRabbit polyclonal to Ephrin B2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic non-phosphopeptide derived from human Ephrin B2 around the phosphorylation site of tyrosine 316 (P-V-YP-I-V).
- HUVEC cells treated with Insulin or EGF.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab75868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 37 kDa).|
FunctionCell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
(Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.
Tissue specificityLung and kidney.
Sequence similaritiesBelongs to the ephrin family.
Contains 1 ephrin RBD (ephrin receptor-binding) domain.
modificationsInducible phosphorylation of tyrosine residues in the cytoplasmic domain.
- Information by UniProt
- EFN B2 antibody
- EFNB 2 antibody
- Efnb2 antibody
All lanes : Anti-Ephrin B2 antibody (ab75868) at 1/500 dilution
Lane 1 : HUVEC cells, untreated with immunising peptide
Lane 2 : HUVEC cells, treated with EGF
Lane 3 : HUVEC cells, treated with Insulin
Predicted band size: 37 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
N.B. Actual band runs higher than predicted due to glycosylation.
This product has been referenced in:
- Protack CD et al. Eph-B4 regulates adaptive venous remodeling to improve arteriovenous fistula patency. Sci Rep 7:15386 (2017). Read more (PubMed: 29133876) »
- Ji YJ et al. EphrinB2 affects apical constriction in Xenopus embryos and is regulated by ADAM10 and flotillin-1. Nat Commun 5:3516 (2014). Read more (PubMed: 24662724) »