Product nameAnti-Ephrin B2 antibody
See all Ephrin B2 primary antibodies
DescriptionRabbit polyclonal to Ephrin B2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rat
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Ephrin B2.
- This antibody gave a positive signal in Human lung and Human kidney tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 36 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionCell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
(Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.
Tissue specificityLung and kidney.
Sequence similaritiesBelongs to the ephrin family.
Contains 1 ephrin RBD (ephrin receptor-binding) domain.
modificationsInducible phosphorylation of tyrosine residues in the cytoplasmic domain.
- Information by UniProt
- EFN B2 antibody
- EFNB 2 antibody
- Efnb2 antibody
All lanes : Anti-Ephrin B2 antibody (ab96264) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Additional bands at: 73 kDa, 86 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The band observed at 32 kDa could potentially be a cleaved form of Ephrin B2 due to the presence of a 27 amino acid signal peptide.
ICC/IF image of ab96264 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96264, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Sivarapatna A et al. Engineered Microvasculature in PDMS Networks Using Endothelial Cells Derived from Human Induced Pluripotent Stem Cells. Cell Transplant 26:1365-1379 (2017). Read more (PubMed: 28901188) »
- Alam SK et al. DNA damage-induced ephrin-B2 reverse signaling promotes chemoresistance and drives EMT in colorectal carcinoma harboring mutant p53. Cell Death Differ 23:707-22 (2016). Read more (PubMed: 26494468) »