Recombinant
RabMAb

Recombinant Anti-Ephrin B2 antibody [EPR10072(B)] (ab150411)

Rabbit recombinant monoclonal Ephrin B2 antibody [EPR10072(B)]. Validated in WB, ICC/IF and tested in Mouse, Rat, Human. Cited in 6 publication(s). Independently reviewed in 3 review(s).

Overview

  • Product name
    Anti-Ephrin B2 antibody [EPR10072(B)]
    See all Ephrin B2 primary antibodies
  • Description
    Rabbit monoclonal [EPR10072(B)] to Ephrin B2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Ephrin B2 aa 50-150. The exact sequence is proprietary.

  • Positive control
    • WB: Recombinant Human Ephrin B2 protein (ab132951), Human fetal kidney, human fetal lung, mouse spleen and rat spleen tissue lysates and 293T cell lysate. ICC/IF: HEK293 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab150411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 36 kDa).
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
      (Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.
    • Tissue specificity
      Lung and kidney.
    • Sequence similarities
      Belongs to the ephrin family.
      Contains 1 ephrin RBD (ephrin receptor-binding) domain.
    • Post-translational
      modifications
      Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • EFN B2 antibody
      • EFNB 2 antibody
      • Efnb2 antibody
      • EFNB2_HUMAN antibody
      • Eph related receptor tyrosine kinase ligand 5 antibody
      • EPH-related receptor tyrosine kinase ligand 5 antibody
      • ephrin B2 antibody
      • Ephrin-B2 antibody
      • EphrinB2 antibody
      • EPLG 5 antibody
      • EPLG5 antibody
      • Htk L antibody
      • HTK ligand antibody
      • HTK-L antibody
      • HTKL antibody
      • LERK 5 antibody
      • LERK-5 antibody
      • LERK5 antibody
      • Ligand of eph related kinase 5 antibody
      • MGC126226 antibody
      • MGC126227 antibody
      • MGC126228 antibody
      • OTTMUSP00000024973 antibody
      see all

    Images

    • All lanes : Anti-Ephrin B2 antibody [EPR10072(B)] (ab150411) at 1/5000 dilution (purified)

      Lane 1 : Human fetal kidney tissue lysate
      Lane 2 : Human fetal lung tissue lysate
      Lane 3 : HEK293 whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 36 kDa
      Observed band size: 50 kDa
      why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST
    • All lanes : Anti-Ephrin B2 antibody [EPR10072(B)] (ab150411) at 1/10000 dilution (purified)

      Lane 1 : Mouse spleen tissue lysate
      Lane 2 : Rat spleen tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 36 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST
    • Immunocytochemistry/Immunofluorescence analysis of A431 (Human epidermoid carcinoma cell line) cells labeling Ephrin B2 with purified ab150411 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: Primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    • All lanes : Anti-Ephrin B2 antibody [EPR10072(B)] (ab150411) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal lung lysate
      Lane 3 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 36 kDa

    • Immunofluorescent analysis of 4% paraformaldehyde-fixed HEK293 cells labeling Ephrin B2 with ab150411 at 1/100 dilution. The cells were permeabilised with 0.1% Triton X. Anti-rabbit Alexa Fluor® 488 (ab150077) at 1/1000 dilution was used as the secondary antibody (green). The confocal image shows membrane and cytoplasmic staining on HEK293 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 and anti-mouse Alexa Fluor® 594 (ab150120) at 1/1000 dilution (red).
      The negative controls are as follows:
      1. ab150411 at 1/1000 dilution followed by anti-mouse Alexa Fluor® 594 (ab150120) at 1/1000 dilution.
      2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by anti-rabbit Alexa Fluor® 488 (ab150077) at 1/1000 dilution.

    References

    This product has been referenced in:
    • Cao C  et al. Bidirectional juxtacrine ephrinB2/Ephs signaling promotes angiogenesis of ECs and maintains self-renewal of MSCs. Biomaterials 172:1-13 (2018). WB . Read more (PubMed: 29709731) »
    • Ge YW  et al. Titanium particle-mediated osteoclastogenesis may be attenuated via bidirectional ephrin-B2/eph-B4 signaling in vitro. Int J Mol Med 42:2031-2041 (2018). Read more (PubMed: 30015911) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Mouse brain)
    Gel Running Conditions
    Reduced Denaturing (5-15%)
    Loading amount
    20 µg
    Specification
    Mouse brain
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Oct 16 2018

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse brain blood vessels)
    Permeabilization
    Yes - 5%Triton X-100
    Specification
    Mouse brain blood vessels
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 35°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Oct 15 2018

    Application
    Flow Cytometry
    Sample
    Mouse Cell (Individualised cells from mice ear tissue.)
    Permeabilization
    No
    Gating Strategy
    (GFP+ Efnb2+) Gatering strategy described in the image.
    Specification
    Individualised cells from mice ear tissue.
    Fixation
    No fixation

    Fernando Jimenez

    Verified customer

    Submitted Apr 19 2016

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