Recombinant
RabMAb

Recombinant Anti-Ephrin B2 antibody [EPR10072(B)] (HRP) (ab201512)

Overview

  • Product name

    Anti-Ephrin B2 antibody [EPR10072(B)] (HRP)
    See all Ephrin B2 primary antibodies
  • Description

    Rabbit monoclonal [EPR10072(B)] to Ephrin B2 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Ephrin B2 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: Recombinant Human Ephrin B2 protein (ab132951), Human fetal kidney lysate and HEK293 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab201512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 36 kDa).
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
      (Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.
    • Tissue specificity

      Lung and kidney.
    • Sequence similarities

      Belongs to the ephrin family.
      Contains 1 ephrin RBD (ephrin receptor-binding) domain.
    • Post-translational
      modifications

      Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • EFN B2 antibody
      • EFNB 2 antibody
      • Efnb2 antibody
      • EFNB2_HUMAN antibody
      • Eph related receptor tyrosine kinase ligand 5 antibody
      • EPH-related receptor tyrosine kinase ligand 5 antibody
      • ephrin B2 antibody
      • Ephrin-B2 antibody
      • EphrinB2 antibody
      • EPLG 5 antibody
      • EPLG5 antibody
      • Htk L antibody
      • HTK ligand antibody
      • HTK-L antibody
      • HTKL antibody
      • LERK 5 antibody
      • LERK-5 antibody
      • LERK5 antibody
      • Ligand of eph related kinase 5 antibody
      • MGC126226 antibody
      • MGC126227 antibody
      • MGC126228 antibody
      • OTTMUSP00000024973 antibody
      see all

    Images

    • All lanes : Anti-Ephrin B2 antibody [EPR10072(B)] (HRP) (ab201512) at 1/5000 dilution

      Lane 1 : Kidney (Human) Tissue Lysate - adult normal tissue
      Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 36 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab201512 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    References

    ab201512 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (skin)
    Permeabilization
    No
    Specification
    skin
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Mar 23 2017

    Application
    ELISA
    Sample
    Human Recombinant protein (EphrinB2-Fc)
    Specification
    EphrinB2-Fc
    Blocking step
    Top Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
    Type
    Sandwich (Capture)

    Abcam user community

    Verified customer

    Submitted Dec 14 2016

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer, PH 6.0
    Specification
    mouse brain
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Mar 03 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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