Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10072(B)] to Ephrin B2 (HRP)
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Product nameAnti-Ephrin B2 antibody [EPR10072(B)] (HRP)
See all Ephrin B2 primary antibodies
DescriptionRabbit monoclonal [EPR10072(B)] to Ephrin B2 (HRP)
Tested applicationsSuitable for: WBmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Ephrin B2 aa 50-150. The exact sequence is proprietary.
- WB: Recombinant Human Ephrin B2 protein (ab132951), Human fetal kidney lysate and HEK293 whole cell lysate.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab201512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 36 kDa).|
FunctionCell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
(Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.
Tissue specificityLung and kidney.
Sequence similaritiesBelongs to the ephrin family.
Contains 1 ephrin RBD (ephrin receptor-binding) domain.
modificationsInducible phosphorylation of tyrosine residues in the cytoplasmic domain.
- Information by UniProt
- EFN B2 antibody
- EFNB 2 antibody
- Efnb2 antibody
All lanes : Anti-Ephrin B2 antibody [EPR10072(B)] (HRP) (ab201512) at 1/5000 dilution
Lane 1 : Kidney (Human) Tissue Lysate - adult normal tissue
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab201512 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab201512 has been referenced in 2 publications.
- Gupta C et al. EphrinB2 expression in prostate adenocarcinoma: Implications for targeted therapy. Pathol Res Pract 216:152967 (2020). PubMed: 32362422
- Li KN et al. Skin vasculature and hair follicle cross-talking associated with stem cell activation and tissue homeostasis. Elife 8:N/A (2019). PubMed: 31343406