Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody (ab193634)

Overview

  • Product name

    Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody
  • Description

    Rabbit polyclonal to Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324)
  • Host species

    Rabbit
  • Specificity

    ab193634 detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at tyrosine 324/311/318 respectivly.

  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Ephrin B1 aa 317-330 (phospho Y324) conjugated to keyhole limpet haemocyanin. Swiss-Prot: P98172/P52799/Q15768.
    Sequence:

    CEKVSGDYGHPVYI

  • Positive control

    • Extracts from HepG2 cells treated with serum. Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab193634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Predicted molecular weight: 47 kDa.

Target

Images

  • All lanes : Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody (ab193634) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells treated with serum
    Lane 2 : Extracts from HepG2 cells treated with serum with antigen-specific peptide

    Predicted band size: 47 kDa

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue sections labeling Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) with ab193634 at a 1/50 dilution. Right image is treated with blocking peptide.

References

ab193634 has not yet been referenced specifically in any publications.

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