Anti-Ephrin B2 (phospho Y316) antibody (ab119323)


  • Product name
    Anti-Ephrin B2 (phospho Y316) antibody
    See all Ephrin B2 primary antibodies
  • Description
    Rabbit polyclonal to Ephrin B2 (phospho Y316)
  • Host species
  • Specificity
    ab119323 recognizes endogenous Ephrin B2 only when phosphorylated at Tyrosine 316. May also react with Ephrin B1 and B3 as the immunogen sequence is also present in these proteins if phophorylated.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide from around the site corresponding to amino acids 314-318 (PVYPIV) of Human Ephrin B2, conjugated to KLH.

  • Positive control
    • HT29 cell lysate



Our Abpromise guarantee covers the use of ab119323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 37 kDa.


  • Function
    Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
    (Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.
  • Tissue specificity
    Lung and kidney.
  • Sequence similarities
    Belongs to the ephrin family.
    Contains 1 ephrin RBD (ephrin receptor-binding) domain.
  • Post-translational
    Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • EFN B2 antibody
    • EFNB 2 antibody
    • Efnb2 antibody
    • EFNB2_HUMAN antibody
    • Eph related receptor tyrosine kinase ligand 5 antibody
    • EPH-related receptor tyrosine kinase ligand 5 antibody
    • ephrin B2 antibody
    • Ephrin-B2 antibody
    • EphrinB2 antibody
    • EPLG 5 antibody
    • EPLG5 antibody
    • Htk L antibody
    • HTK ligand antibody
    • HTK-L antibody
    • HTKL antibody
    • LERK 5 antibody
    • LERK-5 antibody
    • LERK5 antibody
    • Ligand of eph related kinase 5 antibody
    • MGC126226 antibody
    • MGC126227 antibody
    • MGC126228 antibody
    • OTTMUSP00000024973 antibody
    see all


  • All lanes : Anti-Ephrin B2 (phospho Y316) antibody (ab119323) at 1/500 dilution

    Lane 1 : HT29 cell lysate
    Lane 2 : HT29 cells, treated with UV and lysed

    Predicted band size: 37 kDa


This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

The UV light exposure was 15min long. The UV treatment was performed on a clean bench. The UV lamp is fixed in a clean bench without a hood. The distance from cell to lamp is 40cm. The UV output is 13 w/cm2. Culture with serum for 1hr and lyse with RIP...

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We do not know of any tests of rat samples with the anti-Ephrin B2 (phospho Y316) antibody ab119323, but reactivity is predicted, based on 100% identity of the immunogen sequence and the homologous rat Ephrin B2 amino acid sequence. The rat sequen...

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Let me just confirm it is the products Anti-Ephrin B2 antibody (ab96264) and Anti-Ephrin B2 (phospho Y316) antibody (ab119323) which you are interested in?

The anti-Ephrin B2 antibody (ab96264) does indeed measure the total Ephrin B2 content,...

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