Anti-Ephrin B2 (phospho Y316) antibody (ab119323)
Key features and details
- Rabbit polyclonal to Ephrin B2 (phospho Y316)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ephrin B2 (phospho Y316) antibody
See all Ephrin B2 primary antibodies -
Description
Rabbit polyclonal to Ephrin B2 (phospho Y316) -
Host species
Rabbit -
Specificity
ab119323 recognizes endogenous Ephrin B2 only when phosphorylated at Tyrosine 316. May also react with Ephrin B1 and B3 as the immunogen sequence is also present in these proteins if phophorylated. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human Ephrin B2 aa 300-400 (phospho Y316) conjugated to keyhole limpet haemocyanin.
Database link: P52799 -
Positive control
- HT29 cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 37 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 37 kDa. |
Target
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Function
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
(Microbial infection) Acts as a receptor for Hendra virus and Nipah virus. -
Tissue specificity
Lung and kidney. -
Sequence similarities
Belongs to the ephrin family.
Contains 1 ephrin RBD (ephrin receptor-binding) domain. -
Post-translational
modificationsInducible phosphorylation of tyrosine residues in the cytoplasmic domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1948 Human
- Entrez Gene: 306636 Rat
- Omim: 600527 Human
- SwissProt: P52799 Human
- Unigene: 149239 Human
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Alternative names
- EFN B2 antibody
- EFNB 2 antibody
- Efnb2 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab119323 has been referenced in 3 publications.
- Wang T et al. Activation of EphrinB2 Signaling Promotes Adaptive Venous Remodeling in Murine Arteriovenous Fistulae. J Surg Res 262:224-239 (2021). PubMed: 33039109
- Zhu S et al. Bidirectional ephrinB2-EphB4 signaling regulates the osteogenic differentiation of canine periodontal ligament stem cells. Int J Mol Med 45:897-909 (2020). PubMed: 31985015
- Zhang G et al. EphB4 forward signalling regulates lymphatic valve development. Nat Commun 6:6625 (2015). WB . PubMed: 25865237