Product nameAnti-Ephrin B3 antibody
See all Ephrin B3 primary antibodies
DescriptionRabbit polyclonal to Ephrin B3
Tested applicationsSuitable for: ICC/IF, WB, IHCmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Horse, Cow, Cat, Dog, Pig, Macaque monkey
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Ephrin B3.
- This antibody gave a positive signal in the following tissue lysates: Human brain; Mouse brain; Rat brain; Mouse forebrain; Rat forebrain; Mouse Embryonic brain E10; Mouse embryonic brain E14.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab101699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 36 kDa).|
|IHC||Use at an assay dependent concentration. PubMed: 25309991|
FunctionMay play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons.
Tissue specificityHighly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments.
Sequence similaritiesBelongs to the ephrin family.
- Information by UniProt
- EFL 6 antibody
- EFL6 antibody
- EFNB 3 antibody
All lanes : Anti-Ephrin B3 antibody (ab101699) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Forebrain (Mouse) Tissue Lysate
Lane 5 : Rat Forebrain
Lane 6 : E10 Mouse Embryo Brain Tissue Lysate
Lane 7 : E14 Mouse Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 90 seconds
Ephrin B3 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
ICC/IF image of ab101699 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101699, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Efazat G et al. Ephrin B3 interacts with multiple EphA receptors and drives migration and invasion in non-small cell lung cancer. Oncotarget 7:60332-60347 (2016). Read more (PubMed: 27533087) »
- Qu B et al. Testing stem cell therapy in a rat model of inflammatory bowel disease: role of bone marrow stem cells and stem cell factor in mucosal regeneration. PLoS One 9:e107891 (2014). IHC ; Rat . Read more (PubMed: 25309991) »