Overview

  • Product name
  • Description
    Rabbit polyclonal to Ephrin B3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Cow, Cat, Dog, Pig, Macaque monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Ephrin B3.

    Read Abcam's proprietary immunogen policy

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human brain; Mouse brain; Rat brain; Mouse forebrain; Rat forebrain; Mouse Embryonic brain E10; Mouse embryonic brain E14.

Properties

Applications

Our Abpromise guarantee covers the use of ab101699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 36 kDa).
IHC Use at an assay dependent concentration. PubMed: 25309991

Target

  • Function
    May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons.
  • Tissue specificity
    Highly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments.
  • Sequence similarities
    Belongs to the ephrin family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EFL 6 antibody
    • EFL6 antibody
    • EFNB 3 antibody
    • Efnb3 antibody
    • EFNB3_HUMAN antibody
    • EPH related receptor transmembrane ligand ELK L3 antibody
    • EPH related receptor transmembrane ligand ELKL3 antibody
    • EPH related receptor tyrosine kinase ligand 8 antibody
    • EPH-related receptor transmembrane ligand ELK-L3 antibody
    • EPH-related receptor tyrosine kinase ligand 8 antibody
    • Ephrin B3 antibody
    • Ephrin-B3 antibody
    • EphrinB3 antibody
    • EPLG 8 antibody
    • EPLG8 antibody
    • LERK 8 antibody
    • LERK-8 antibody
    • LERK8 antibody
    see all

Images

  • All lanes : Anti-Ephrin B3 antibody (ab101699) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Forebrain (Mouse) Tissue Lysate
    Lane 5 : Rat Forebrain
    Lane 6 : E10 Mouse Embryo Brain Tissue Lysate
    Lane 7 : E14 Mouse Embryo Brain Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 90 seconds


    Ephrin B3 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • ICC/IF image of ab101699 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101699, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Efazat G  et al. Ephrin B3 interacts with multiple EphA receptors and drives migration and invasion in non-small cell lung cancer. Oncotarget 7:60332-60347 (2016). Read more (PubMed: 27533087) »
  • Qu B  et al. Testing stem cell therapy in a rat model of inflammatory bowel disease: role of bone marrow stem cells and stem cell factor in mucosal regeneration. PLoS One 9:e107891 (2014). IHC ; Rat . Read more (PubMed: 25309991) »
See all 2 Publications for this product

Customer reviews and Q&As

Filter by Application

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Application
Flow Cytometry
Sample
Mouse Cell (small cell lung cancer cell line 16T)
Permeabilization
No
Gating Strategy
single cells with FSC and SSC, live cells with low DAPI, positive/negative cells with PI filter set, anti-CD24 as positive control
Specification
small cell lung cancer cell line 16T
Preparation
Cell harvesting/tissue preparation method: Cell harvesting: cells grow as floating spheres. Collect by spinning, dissociate spheres with TrypLE, single cell through 40um cell strainer
Sample buffer: PBS + 10% FBS
Fixation
none

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Submitted Jul 26 2018

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