Product nameAnti-Ephrin B3 antibody
See all Ephrin B3 primary antibodies
DescriptionRabbit polyclonal to Ephrin B3
SpecificityThis antibody detects endogenous levels of total Ephrin B3 protein.
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
A synthesized peptide derived from human Ephrin B3.
- Human brain tissue and extracts from SKOV3 cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab53063 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionMay play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons.
Tissue specificityHighly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments.
Sequence similaritiesBelongs to the ephrin family.
- Information by UniProt
- EFL 6 antibody
- EFL6 antibody
- EFNB 3 antibody
All lanes : Anti-Ephrin B3 antibody (ab53063) at 1/500 dilution
Lane 1 : Extracts from SKOV3 cells
Lane 2 : Extracts from SKOV3 cells treated with peptide
Predicted band size: 36 kDa
Observed band size: 36 kDa
Immunohistochemical analysis of paraffin-embedded human brain tissue using ab53063 at a 1:50 dilution.
Left image un-treated.
Right image treated with peptide.
ICC/IF image of ab53063 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53063, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab53063 has not yet been referenced specifically in any publications.