Anti-EPHX2 antibody [EPR10491(B)] (ab155280)
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Overview
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Product nameAnti-EPHX2 antibody [EPR10491(B)]
See all EPHX2 primary antibodies -
DescriptionRabbit monoclonal [EPR10491(B)] to EPHX2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide, corresponding to residues in human EPHX2 was used as an immunogen (UniProt: P34913).
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Positive control
- 293T, MCF7, Human fetal liver and fetal kidney lysates; Permeabilized 293T cells.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR10491(B)
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 63 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionActs on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. Has low phosphatase activity.
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Sequence similaritiesBelongs to the AB hydrolase superfamily. Epoxide hydrolase family.
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DomainThe N-terminal domain has phosphatase activity. The C-terminal domain has epoxide hydrolase activity.
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Post-translational
modificationsThe N-terminus is blocked.
The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation. -
Cellular localizationCytoplasm. Peroxisome.
- Information by UniProt
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Database links
- Entrez Gene: 2053 Human
- Entrez Gene: 13850 Mouse
- Omim: 132811 Human
- SwissProt: P34913 Human
- SwissProt: P34914 Mouse
- Unigene: 212088 Human
- Unigene: 15295 Mouse
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Alternative names
- Bifunctional epoxide hydrolase 2 antibody
- CEH antibody
- Cytosolic epoxide hydrolase antibody
see all
Images
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![Western blot - Anti-EPHX2 antibody [EPR10491(B)] (ab155280)](https://www.abcam.com/ps/products/155/ab155280/Images/EPHX2-Primary-antibodies-ab155280-1.jpg)
Western blot - Anti-EPHX2 antibody [EPR10491(B)] (ab155280)All lanes : Anti-EPHX2 antibody [EPR10491(B)] (ab155280) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 63 kDa -
![Flow Cytometry - Anti-EPHX2 antibody [EPR10491(B)] (ab155280)](https://www.abcam.com/ps/products/155/ab155280/Images/EPHX2-Primary-antibodies-ab155280-2.jpg)
Flow Cytometry - Anti-EPHX2 antibody [EPR10491(B)] (ab155280)Flow cytometric analysis of permeabilized 293T cells, labeling EPHX2 with ab155280 at 1/10 (red) compared to a rabbit IgG (negative) (green).
Datasheets and documents
References
ab155280 has not yet been referenced specifically in any publications.
