Overview

  • Product name
    Anti-EPHX2 antibody [EPR10491(B)]
    See all EPHX2 primary antibodies
  • Description
    Rabbit monoclonal [EPR10491(B)] to EPHX2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in human EPHX2 was used as an immunogen (UniProt: P34913).

  • Positive control
    • 293T, MCF7, Human fetal liver and fetal kidney lysates; Permeabilized 293T cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Associated products

Applications

Our Abpromise guarantee covers the use of ab155280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 63 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      Acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. Has low phosphatase activity.
    • Sequence similarities
      Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.
    • Domain
      The N-terminal domain has phosphatase activity. The C-terminal domain has epoxide hydrolase activity.
    • Post-translational
      modifications
      The N-terminus is blocked.
      The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation.
    • Cellular localization
      Cytoplasm. Peroxisome.
    • Information by UniProt
    • Database links
    • Alternative names
      • Bifunctional epoxide hydrolase 2 antibody
      • CEH antibody
      • Cytosolic epoxide hydrolase antibody
      • EPHX2 antibody
      • Epoxide hydratase antibody
      • Epoxide hydrolase 2 antibody
      • Epoxide hydrolase 2 cytoplasmic antibody
      • epoxide hydrolase 2, cytosolic antibody
      • Epoxide hydrolase soluble antibody
      • HYES_HUMAN antibody
      • SEH antibody
      • Soluble epoxide hydrolase antibody
      see all

    Images

    • All lanes : Anti-EPHX2 antibody [EPR10491(B)] (ab155280) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : Human fetal liver lysate
      Lane 4 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 63 kDa

    • Flow cytometric analysis of permeabilized 293T cells, labeling EPHX2 with ab155280 at 1/10 (red) compared to a rabbit IgG (negative) (green).

    References

    ab155280 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver)
    Gel Running Conditions
    Non-reduced Denaturing (12% gel)
    Loading amount
    30 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

    Abcam user community

    Verified customer

    Submitted Jun 20 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up