Recombinant Anti-EPHX2 antibody [EPR10491(B)] (ab155280)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10491(B)] to EPHX2
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
Overview
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Product name
Anti-EPHX2 antibody [EPR10491(B)]
See all EPHX2 primary antibodies -
Description
Rabbit monoclonal [EPR10491(B)] to EPHX2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human EPHX2 aa 1-100. The exact sequence is proprietary.
Database link: P34913 -
Positive control
- 293T, MCF7, Human fetal liver and fetal kidney lysates; Permeabilized 293T cells.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10491(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab155280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 63 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. Has low phosphatase activity. -
Sequence similarities
Belongs to the AB hydrolase superfamily. Epoxide hydrolase family. -
Domain
The N-terminal domain has phosphatase activity. The C-terminal domain has epoxide hydrolase activity. -
Post-translational
modificationsThe N-terminus is blocked.
The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation. -
Cellular localization
Cytoplasm. Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 2053 Human
- Entrez Gene: 13850 Mouse
- Omim: 132811 Human
- SwissProt: P34913 Human
- SwissProt: P34914 Mouse
- Unigene: 212088 Human
- Unigene: 15295 Mouse
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Alternative names
- Bifunctional epoxide hydrolase 2 antibody
- CEH antibody
- Cytosolic epoxide hydrolase antibody
see all
Images
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![Western blot - Anti-EPHX2 antibody [EPR10491(B)] (ab155280)](https://www.abcam.com/ps/products/155/ab155280/Images/ab155280-162357-anti-ephx2-antibody-epr10491b-western-blot.jpg)
Western blot - Anti-EPHX2 antibody [EPR10491(B)] (ab155280)All lanes : Anti-EPHX2 antibody [EPR10491(B)] (ab155280) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 63 kDa -
![Flow Cytometry - Anti-EPHX2 antibody [EPR10491(B)] (ab155280)](https://www.abcam.com/ps/products/155/ab155280/Images/ab155280-162356-anti-ephx2-antibody-epr10491b-flow-cytometry.jpg)
Flow Cytometry - Anti-EPHX2 antibody [EPR10491(B)] (ab155280)Flow cytometric analysis of permeabilized 293T cells, labeling EPHX2 with ab155280 at 1/10 (red) compared to a rabbit IgG (negative) (green). -
![Anti-EPHX2 antibody [EPR10491(B)] (ab155280)](https://www.abcam.com/ps/products/155/ab155280/Images/ab155280-3-benefits-of-recombinant-antibodies.png)
Anti-EPHX2 antibody [EPR10491(B)] (ab155280)
Datasheets and documents
References (1)
ab155280 has been referenced in 1 publication.
- Yang HH et al. A COX-2/sEH dual inhibitor PTUPB alleviates lipopolysaccharide-induced acute lung injury in mice by inhibiting NLRP3 inflammasome activation. Theranostics 10:4749-4761 (2020). PubMed: 32308747
