Recombinant
RabMAb

Recombinant Anti-EPHX2 antibody [EPR10491(B)] (ab155280)

Reviews (1) Submit a question

Overview

  • Product name

    Anti-EPHX2 antibody [EPR10491(B)]
    See all EPHX2 primary antibodies

  • Description

    Rabbit monoclonal [EPR10491(B)] to EPHX2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human EPHX2 aa 1-100. The exact sequence is proprietary.
    Database link: P34913

  • Positive control

    • 293T, MCF7, Human fetal liver and fetal kidney lysates; Permeabilized 293T cells.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab155280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 63 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function

      Acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. Has low phosphatase activity.

    • Sequence similarities

      Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.

    • Domain

      The N-terminal domain has phosphatase activity. The C-terminal domain has epoxide hydrolase activity.

    • Post-translational
      modifications

      The N-terminus is blocked.
      The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation.

    • Cellular localization

      Cytoplasm. Peroxisome.
    • Information by UniProt

    • Database links

      • Alternative names

        • Bifunctional epoxide hydrolase 2 antibody
        • CEH antibody
        • Cytosolic epoxide hydrolase antibody
        • EPHX2 antibody
        • Epoxide hydratase antibody
        • Epoxide hydrolase 2 antibody
        • Epoxide hydrolase 2 cytoplasmic antibody
        • epoxide hydrolase 2, cytosolic antibody
        • Epoxide hydrolase soluble antibody
        • HYES_HUMAN antibody
        • SEH antibody
        • Soluble epoxide hydrolase antibody
        see all

      Images

      • Western blot - Anti-EPHX2 antibody [EPR10491(B)] (ab155280)
        Western blot - Anti-EPHX2 antibody [EPR10491(B)] (ab155280)
        All lanes : Anti-EPHX2 antibody [EPR10491(B)] (ab155280) at 1/10000 dilution

        Lane 1 : 293T cell lysate
        Lane 2 : MCF7 cell lysate
        Lane 3 : Human fetal liver lysate
        Lane 4 : Human fetal kidney lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 63 kDa

      • Flow Cytometry - Anti-EPHX2 antibody [EPR10491(B)] (ab155280)
        Flow Cytometry - Anti-EPHX2 antibody [EPR10491(B)] (ab155280)
        Flow cytometric analysis of permeabilized 293T cells, labeling EPHX2 with ab155280 at 1/10 (red) compared to a rabbit IgG (negative) (green).

      References

      ab155280 has not yet been referenced specifically in any publications.

      Publishing research using ab155280? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Western blot abreview for Anti-EPHX2 antibody [EPR10491(B)]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Liver)
      Gel Running Conditions
      Non-reduced Denaturing (12% gel)
      Loading amount
      30 µg
      Specification
      Liver
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 20 2017

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Sign up