Recombinant Anti-EPHX2 antibody [EPR10491(B)] (ab155280)
![Western blot - Anti-EPHX2 antibody [EPR10491(B)] (ab155280)](https://www.abcam.com/ps/products/155/ab155280/Images/ab155280-162357-anti-ephx2-antibody-epr10491b-western-blot.jpg "Western blot - Anti-EPHX2 antibody [EPR10491(B)] (ab155280)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10491(B)] to EPHX2
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-EPHX2 antibody [EPR10491(B)]
See all EPHX2 primary antibodies -
Description
Rabbit monoclonal [EPR10491(B)] to EPHX2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human EPHX2 aa 1-100. The exact sequence is proprietary.
Database link: P34913 -
Positive control
- 293T, MCF7, Human fetal liver and fetal kidney lysates; Permeabilized 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10491(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 63 kDa.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Predicted molecular weight: 63 kDa. |
Target
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Function
Acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. Has low phosphatase activity. -
Sequence similarities
Belongs to the AB hydrolase superfamily. Epoxide hydrolase family. -
Domain
The N-terminal domain has phosphatase activity. The C-terminal domain has epoxide hydrolase activity. -
Post-translational
modificationsThe N-terminus is blocked.
The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation. -
Cellular localization
Cytoplasm. Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 2053 Human
- Entrez Gene: 13850 Mouse
- Omim: 132811 Human
- SwissProt: P34913 Human
- SwissProt: P34914 Mouse
- Unigene: 212088 Human
- Unigene: 15295 Mouse
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Alternative names
- Bifunctional epoxide hydrolase 2 antibody
- CEH antibody
- Cytosolic epoxide hydrolase antibody
see all
Images
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Western blot - Anti-EPHX2 antibody [EPR10491(B)] (ab155280)All lanes : Anti-EPHX2 antibody [EPR10491(B)] (ab155280) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 63 kDa -
![Flow Cytometry (Intracellular) - Anti-EPHX2 antibody [EPR10491(B)] (ab155280)](https://www.abcam.com/ps/products/155/ab155280/Images/ab155280-162356-anti-ephx2-antibody-epr10491b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-EPHX2 antibody [EPR10491(B)] (ab155280)Intracellular flow cytometric analysis of permeabilized 293T cells, labeling EPHX2 with ab155280 at 1/10 (red) compared to a rabbit IgG (negative) (green).
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![Anti-EPHX2 antibody [EPR10491(B)] (ab155280)](https://www.abcam.com/ps/products/155/ab155280/Images/ab155280-3-benefits-of-recombinant-antibodies.png)
Anti-EPHX2 antibody [EPR10491(B)] (ab155280)
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab155280 has been referenced in 4 publications.
- Yang HH et al. A COX-2/sEH dual inhibitor PTUPB alleviates lipopolysaccharide-induced acute lung injury in mice by inhibiting NLRP3 inflammasome activation. Theranostics 10:4749-4761 (2020). PubMed: 32308747
- Li J et al. Silencing of soluble epoxide hydrolase 2 gene reduces H2O2-induced oxidative damage in rat intestinal epithelial IEC-6 cells via activating PI3K/Akt/GSK3ß signaling pathway. Cytotechnology 72:23-36 (2020). PubMed: 31907700
- Liu G et al. Inactivation of Cys674 in SERCA2 increases BP by inducing endoplasmic reticulum stress and soluble epoxide hydrolase. Br J Pharmacol 177:1793-1805 (2020). PubMed: 31758704
- Liang Z et al. 1-Trifluoromethoxyphenyl-3-(1-propionylpiperidin-4-yl) Urea, a Selective and Potent Dual Inhibitor of Soluble Epoxide Hydrolase and p38 Kinase Intervenes in Alzheimer's Signaling in Human Nerve Cells. ACS Chem Neurosci 10:4018-4030 (2019). PubMed: 31378059