Product nameAnti-EPI64 antibody - C-terminal
See all EPI64 primary antibodies
DescriptionRabbit polyclonal to EPI64 - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Cow, Cat, Pig, Rhesus monkey, Gorilla, Orangutan
- Whole cell lysate from HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH3T3 cells.
Previously labelled as TBC1D10A.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab194945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 57 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionActs as GTPase-activating protein for RAB27A, but not for RAB2A, RAB3A, nor RAB4A.
Sequence similaritiesContains 1 Rab-GAP TBC domain.
modificationsExists in both phosphorylated and non-phosphorylated state.
Cellular localizationCell projection > microvillus. Localizes to the microvilli-rich region of the syncytiotrophoblast. In melanocytes, located at the periphery of cells.
- Information by UniProt
- dJ130H16.1 antibody
- dJ130H16.2 antibody
- EBP50 PDZ interactor of 64 kD antibody
All lanes : Anti-EPI64 antibody - C-terminal (ab194945) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa
Lane 2 : Whole cell lysate from 293T
Lane 3 : Whole cell lysate from Jurkat
Lane 4 : Whole cell lysate from TCMK-1
Lane 5 : Whole cell lysate from NIH 3T3
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 3 minutes
Detection of Human EPI64 by Western Blot of Immunoprecipitates.
Lane 1: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. ab194945 was used for IP at 6 μg per reaction, and at 0.4 μg/ml for blotting.
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab194945 has not yet been referenced specifically in any publications.