For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Histone H3 mutations

Histone H3 mutations in cancer

Related

  • Histone modifications
    • Histone modifications antibodies
      • HDAC antibodies
      • Cancer epigenetics guide
        • Cancer epigenetics poster
          • Chromatin accessibility
            • ChIP guide
              • DNA methylation
                • RNA modifications
                  • Epigenetic modifications poster
                    • Polycomb and chromatin remodeling resources
                      • Guide to chromatin remodeling complexes
                        • Chromatin remodeling research tools

                          Your guide to studying histone H3 mutations in cancer.

                          ​Updated November 21, 2022

                          We've put together a quick overview of the most common histone mutations affecting histone 3 (H3) - H3K27M, H3K36M, H3G34 (W/V/R) - so you can easily find the right tools to study your histone mutant.

                          Contents

                          • Histone mutations in cancer
                          • Find the right marker for your histone H3 mutant
                            • H3K27M
                            • H3K36M
                            • H3G34W
                            • H3G34V
                            • H3G34R
                          • Explore our histone H3 mutant antibody panels
                          • References

                          Histone mutations in cancer​

                          Recurrent histone mutations have been recently described in various cancer types, including brain tumors, chondroblastoma, giant cell tumors of bone, and leukemia. Current research suggests that histone mutations alter the epigenetic landscape, but underlying mechanisms are not completely understood.

                          The identification of widespread recurrent driver mutations in histones has raised significant interest in the development of novel targeted epigenetic therapies for histone mutant tumors, as well as biomarkers for diagnostics and monitoring of drug response.

                          Here we review the five common histone mutations affecting H3 - H3K27M, H3K36M, and H3G34 (W/V/R) (Figure 1) - and recommend our best recombinant monoclonal antibodies to study these targets.

                          Find the right marker for your histone H3 mutant

                          H3K27M


                          IHC of paraffin-embedded mouse brain tissue labeling H3K27M with ab190631 at 1/500 dilution followed by Discovery UltraMap anti-rabbit (HRP).

                          H3K27M mutations are found in pediatric brain tumors and adult cancers, such as acute myeloid leukemia, melanoma, and glioma1. H3K27M acts as a suppressor of the PCR2 complex, leading to a global reduction of H3K27 di- and trimethylation levels (H3K27me2 and H3K27me3)2.

                          We recommend Recombinant anti-H3K27M antibody - ChIP Grade (ab190631)

                          Applications: WB, Indirect ELISA, IHC-P, ICC/IF, IP, ChIP

                          Browse all H3K27M products

                          Related targets: EZH2, SUZ12, EED, p53, PDFRA, FGFR1, ACVR1, BCOR, H3K27me2, H3K27me3


                          H3K36M


                          IHC of paraffin-embedded human chondroblastoma tissue labeling H3K36M with ab256384 at 1/4000 dilution followed by a goat anti-rabbit IgG H&L (HRP).

                          H3K36M is a potential oncogenic driver mutation in chondroblastomas; also found in pediatric soft tissue sarcomas and HNSCC. 

                          Similar to H3K27M, the H3K36M mutation suppresses the methyltransferase activity of SETD2 and NSD family proteins and leads to the global reduction of H3K36 di- and trimethylation levels3. 

                          We recommend Recombinant anti-H3K36M antibody (ab256384)

                          Applications: IP, WB, IHC-P, ICC/IF, Flow Cyt, Indirect ELISA.

                          Browse all H3K36M products

                          Related targets: SETD2, NSD1, NSD2, H3K36me2, H3K36me3.

                          H3G34 (W/V/R)

                          Histone mutations at H3G34 include substitutions of glycine 34 with tryptophan (G34W), arginine (G34R), or valine (G34V). Several studies indicate that the replacement of H3G34 with any other amino acid residue blocks SETD2 binding and thereby may hinder the methylation of H3K361. 

                          H3G34W