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Histone modification antibodies

Related

  • Histone modification
    • Epigenetic modifications poster
      • ChIP guide
        • X-ChIP protocol
          • ChIP protocols
            • Epigenetic inhibitors
              • Histone H3 mutations in cancer

                Get specificity and reproducibility for your histone modification experiments with monoclonal antibodies.

                Histone proteins undergo post-translational modification (PTM), which impacts their interactions with DNA. Together, these histone modifications make up the histone code, which dictates the transcriptional state of the local genomic region. Examining histone modifications can reveal gene activation states, locations of promoters, enhancers, and other gene regulatory elements. Detection of histone modifications can be done using antibody-based techniques such as IHC/ICC, ChIP-Seq, ChIP-PCR, and many other methods.

                Antibody-based detection of histone modifications

                Get sensitive, easy-to-interpret results every time with highly specific monoclonal antibodies to histone modifications. Recombinant monoclonal antibodies eliminate batch-to-batch variation to give you a consistent antibody and reproducible results throughout your project. 

                Watch here to find out how how using a monoclonal antibody can give you reproducibility in your ChIP-Seq experiments.

                ​

                Polyclonal vs monoclonal antibodies

                Monoclonal antibodies specifically detect one epitope on the protein of interest. They produce more reproducible results than a polyclonal and are less likely to cross-react with other proteins. For histone modifications it makes sense to use a monoclonal antibody binding only to the modification of interest to achieve a consistent, reproducible result.

                Polyclonal antibodies are composed of a mixture of antibodies that represents the natural immune response to an antigen. This mixture of antibodies detects a range of epitopes on a protein and consequently they are prone to a higher risk of lot-to-lot variability than monoclonal antibodies.


                Validation of histone modification antibodies

                Many of our histone modification antibodies are screened by peptide array to select antibodies that do not cross-react with other histone modifications. Our array covers 501 peptides, including single and multiple modifications, unmodified controls, and histone variants.

                Our recombinant monoclonal histone modification antibodies are also routinely validated using several applications including

                • ChIP
                • IHC/ICC
                • Western blot
                • Dot blot

                Find out more about our validation methods here.


                Monoclonal histone modification antibodies


                ModificationFunction Rabbit monoclonal antibodyMouse monoclonal antibody
                H3K4me1Activationab176877​--
                H3K4me3Activationab213224​ab1012​
                H3K9acActivationab32129 ​ab12179​
                H3K27acActivationab177178--
                H3K9me2Repressionab32521ab1220
                H3K9me3Repressionab176916--
                H3K27me3​Repressionab192985--
                ɣH2A.XDNA damageab81299​ab26350​
                H3S10pDNA replicationab177218​ab14955​

                See the full range of monoclonal histone modification antibodies here

                Find out more about histone modifications within our epigenetics guide.






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