Product nameAnti-epithelial Sodium Channel gamma antibody
See all epithelial Sodium Channel gamma primary antibodies
DescriptionRabbit polyclonal to epithelial Sodium Channel gamma
SpecificityDetects recombinant rat gamma-epithelial sodium channel (gamma ENaC).
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Human, Xenopus laevis, Non human primates, Amphibian
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab3468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/1000. Can be blocked with epithelial Sodium Channel gamma peptide (ab4990).|
|IP||Use at an assay dependent concentration. This antibody specifically binds to the unglycosylated, ~75 kDa recombinant rat gamma ENaC protein.|
FunctionSodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
Involvement in diseaseDefects in SCNN1G are a cause of Liddle syndrome (LIDDS) [MIM:177200]. It is an autosomal dominant disorder characterized by pseudoaldosteronism and hypertension associated with hypokalemic alkalosis. The disease is caused by constitutive activation of the renal epithelial sodium channel.
Defects in SCNN1G are the cause of bronchiectasis with or without elevated sweat chloride type 3 (BESC3) [MIM:613071]. A debilitating respiratory disease characterized by chronic, abnormal dilatation of the bronchi and other cystic fibrosis-like symptoms in the absence of known causes of bronchiectasis (cystic fibrosis, autoimmune diseases, ciliary dyskinesia, common variable immunodeficiency, foreign body obstruction). Clinical features include sub-normal lung function, sinopulmonary infections, chronic productive cough, excessive sputum production, and elevated sweat chloride in some cases.
Sequence similaritiesBelongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1G subfamily.
modificationsPhosphorylated on serine and threonine residues.
Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation.
Cellular localizationApical cell membrane. Apical membrane of epithelial cells.
- Information by UniProt
- Amiloride sensitive epithelial sodium channel gamma subunit antibody
- Amiloride sensitive sodium channel subunit gamma antibody
- Amiloride-sensitive sodium channel subunit gamma antibody
All lanes : Anti-epithelial Sodium Channel gamma antibody (ab3468) at 1/2000 dilution
Lane 1 : H441 whole cell lysate (treated with dexamethasone)
Lane 2 : H441 whole cell lysate (treated with dexamethasone) with Immunizing peptide
Lysates/proteins at 50 µg per lane.
All lanes : HRP conjugated donkey anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 90-95 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible non-specific binding)
Exposure time: 15 seconds
ab3468 staining epithelial Sodium Channel gamma in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% Donkey serum for 2 hours at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/300 in KPBS-BT2) for 12 hours at 4°C. An undiluted HRP-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.
This product has been referenced in:
- Sesma JI et al. SPX-101 is stable in and retains function after exposure to cystic fibrosis sputum. J Cyst Fibros N/A:N/A (2018). Read more (PubMed: 29936069) »
- Li Y et al. Novel mechanisms for crotonaldehyde-induced lung edema. Oncotarget 8:83509-83522 (2017). Read more (PubMed: 29137360) »