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    epm2alaforin-antibody-ab129321.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Phosphatases
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Anti-EPM2A/Laforin antibody (ab129321)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPM2A/Laforin antibody (ab129321)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPM2A/Laforin antibody (ab129321)
  • Western blot - Anti-EPM2A/Laforin antibody (ab129321)

Key features and details

  • Goat polyclonal to EPM2A/Laforin
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

View more associated products

Overview

  • Product name

    Anti-EPM2A/Laforin antibody
    See all EPM2A/Laforin primary antibodies
  • Description

    Goat polyclonal to EPM2A/Laforin
  • Host species

    Goat
  • Specificity

    ab129321 is expected to recognize isoform 1 (NP_005661.1) only.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Dog, Pig, Xenopus laevis, a wide range of other species
  • Immunogen

    Synthetic peptide corresponding to Human EPM2A/Laforin aa 131-144 (internal sequence) (Cysteine residue).
    Sequence:

    C-EATGHTNEMKHTTD


    Database link: NP_005661.1
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human brain, cerebellum and heart tissues; Human Cerebellum lysate.
  • General notes

     This product was previously labelled as EPM2A', 'EPM2A, isoform 9

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Phosphatases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 37 kDa.
Notes
IHC-P
Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 37 kDa.

Target

  • Function

    Dual specificity protein phosphatase. May be involved in the control of glycogen metabolism, particularly in monitoring for and preventing the formation of poorly branched glycogen molecules (polyglucosans). Acts as a scaffold protein to facilitate PPP1R3C/PTG ubiquitination by NHLRC1/malin. Forms a complex with NHLRC1/malin and HSP70 and this complex suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Isoform 2, an inactive phosphatase, could function as a dominant-negative regulator for the phosphatase activity of isoform 1.
  • Tissue specificity

    Expressed in heart, skeletal muscle, kidney, pancreas and brain. Isoform 4 is also expressed in the placenta.
  • Involvement in disease

    Defects in EPM2A are a cause of progressive myoclonic epilepsy type 2 (EPM2) [MIM:254780]; also known as Lafora disease. EPM2 is an autosomal recessive and severe form of adolescent-onset progressive epilepsy. Typically, as seizures increase in frequency, cognitive function declines towards dementia, and affected individuals die usually within 10 years after onset. EPM2 occurs worldwide, but it is particularly common in the mediterranean countries of southern Europe and northern Africa, in southern India and in the Middle East. At the cellular level, it is characterized by accumulation of starch-like polyglucosans called Lafora bodies (LBs) that are most abundant in organs with the highest glucose metabolism: brain, heart, liver and skeletal muscle. Among other conditions involving polyglucosans, EPM2 is unique in that the inclusions are in neuronal dendrites but not axons and the forming polyglucosan fibrils are associated with the endoplasmic reticulum.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family.
    Contains 1 CBM20 (carbohydrate binding type-20) domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications

    Polyubiquitinated by NHLRC1/malin.
    Phosphorylation on Ser-25 by AMPK affects the phosphatase activity of the enzyme and its ability to homodimerize and interact with NHLRC1, PPP1R3C or PRKAA2.
  • Cellular localization

    Cytoplasm; Cytoplasm. Nucleus; Endoplasmic reticulum. Cell membrane. Nucleus. Also found in the nucleus; Endoplasmic reticulum. Cell membrane. Primarily associated with polyribosomes at the endoplasmic reticulum, also found at the plasma membrane and Cytoplasm. Under glycogenolytic conditions localizes to the nucleus.
  • Target information above from: UniProt accession O95278 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 484022 Dog
    • Entrez Gene: 7957 Human
    • Omim: 607566 Human
    • SwissProt: Q1M199 Dog
    • SwissProt: O95278 Human
    • Unigene: 486696 Human
    • Alternative names

      • Epilepsy progressive myoclonus type 2 Lafora disease (laforin) antibody
      • Epilepsy progressive myoclonus type 2A Lafora disease (laforin) antibody
      • EPM2 antibody
      • Epm2a antibody
      • Epm2a gene antibody
      • EPM2A_HUMAN antibody
      • Lafora PTPase antibody
      • Laforin antibody
      • LAFPTPase antibody
      • LD antibody
      • LDE antibody
      • MELF antibody
      • RP3-466P17.2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPM2A/Laforin antibody (ab129321)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPM2A/Laforin antibody (ab129321)

      ab129321 at 3.75 µg/ml staining EPM2A/Laforin in Formalin-Fixed, Paraffin-Embedded Human brain, cerebellum tissue by immunohistochemistry.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPM2A/Laforin antibody (ab129321)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPM2A/Laforin antibody (ab129321)

      ab129321 at 3.75 µg/ml staining EPM2A/Laforin in Formalin-Fixed, Paraffin-Embedded Human heart tissue by immunohistochemistry.

    • Western blot - Anti-EPM2A/Laforin antibody (ab129321)
      Western blot - Anti-EPM2A/Laforin antibody (ab129321)
      Anti-EPM2A/Laforin antibody (ab129321) at 0.1 µg/ml + Human Cerebellum lysate in RIPA buffer at 35 µg

      Developed using the ECL technique.

      Predicted band size: 37 kDa

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab129321? Please let us know so that we can cite the reference in this datasheet.

    ab129321 has not yet been referenced specifically in any publications.

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