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Cardiovascular Angiogenesis Growth Factors Other
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Anti-EPO antibody (ab226956)

  • Datasheet
  • SDS
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Western blot - Anti-EPO antibody (ab226956)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO antibody (ab226956)
  • Western blot - Anti-EPO antibody (ab226956)
  • ELISA - Anti-EPO antibody (ab226956)

Key features and details

  • Rabbit polyclonal to EPO
  • Suitable for: IHC-P, WB, ELISA
  • Reacts with: Rat, Human, Recombinant fragment
  • Isotype: IgG

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Protein
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Recombinant Human EPO protein (ab155618)
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-EPO antibody
    See all EPO primary antibodies
  • Description

    Rabbit polyclonal to EPO
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity

    Reacts with: Rat, Human, Recombinant fragment
  • Immunogen

    Recombinant fragment within Human EPO (internal sequence). The exact sequence is proprietary.
    Database link: P01588

  • Positive control

    • IHC-P: Rat liver tissue. WB: Human liver tissue extract.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • Other
    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Stem Cells
    • Hematopoietic Progenitors
    • Intracellular Molecules
    • Cardiovascular
    • Blood
    • Erythrocytes
    • Developmental Biology
    • Organogenesis
    • Hematopoietic system development
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human EPO protein (ab155618)

Applications

Our Abpromise guarantee covers the use of ab226956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/5000 - 1/20000.
ELISA 1/1000 - 1/10000.

Target

  • Relevance

    Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
  • Cellular localization

    Secreted
  • Database links

    • Entrez Gene: 2056 Human
    • Entrez Gene: 24335 Rat
    • Omim: 133170 Human
    • SwissProt: P01588 Human
    • SwissProt: P29676 Rat
    • Unigene: 2303 Human
    • Unigene: 11365 Rat
    • Alternative names

      • EP antibody
      • EPO alpha antibody
      • EPO antibody
      • Epoetin antibody
      • Erythropoetin antibody
      • Erythropoietin precursor antibody
      • MVCD2 antibody
      see all

    Images

    • Western blot - Anti-EPO antibody (ab226956)
      Western blot - Anti-EPO antibody (ab226956)
      Anti-EPO antibody (ab226956) at 1/10000 dilution + recombinant human EPO protein (with glycosylation) expressed in CHO cells at 0.1 µg


      12% SDS-PAGE gel.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO antibody (ab226956)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO antibody (ab226956)

      Paraffin-embedded rat liver tissue stained for EPO using ab226956 at 1/400 dilution in immunohistochemical analysis.

    • Western blot - Anti-EPO antibody (ab226956)
      Western blot - Anti-EPO antibody (ab226956)
      Anti-EPO antibody (ab226956) at 1/500 dilution + Human liver tissue extract at 30 µg


      12% SDS-PAGE gel.

    • ELISA - Anti-EPO antibody (ab226956)
      ELISA - Anti-EPO antibody (ab226956)

      An ELISA plate is coated with 50 μL of human EPO recombinant protein at concentration ranged from 0.125 μg/mL to 4 μg/mL. The coated protein is detected with EPO antibody at 1 μg/mL.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab226956? Please let us know so that we can cite the reference in this datasheet.

    ab226956 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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