Anti-EPO antibody (ab226956)
Key features and details
- Rabbit polyclonal to EPO
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Rat, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-EPO antibody
See all EPO primary antibodies -
Description
Rabbit polyclonal to EPO -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ELISAmore details -
Species reactivity
Reacts with: Rat, Human, Recombinant fragment -
Immunogen
Recombinant fragment within Human EPO (internal sequence). The exact sequence is proprietary.
Database link: P01588 -
Positive control
- IHC-P: Rat liver tissue. WB: Human liver tissue extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab226956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/5000 - 1/20000. | |
| ELISA | 1/1000 - 1/10000. |
Target
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Relevance
Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 2056 Human
- Entrez Gene: 24335 Rat
- Omim: 133170 Human
- SwissProt: P01588 Human
- SwissProt: P29676 Rat
- Unigene: 2303 Human
- Unigene: 11365 Rat
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Alternative names
- EP antibody
- EPO alpha antibody
- EPO antibody
see all
Images
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Western blot - Anti-EPO antibody (ab226956)Anti-EPO antibody (ab226956) at 1/10000 dilution + recombinant human EPO protein (with glycosylation) expressed in CHO cells at 0.1 µg
12% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO antibody (ab226956)Paraffin-embedded rat liver tissue stained for EPO using ab226956 at 1/400 dilution in immunohistochemical analysis.
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Western blot - Anti-EPO antibody (ab226956)Anti-EPO antibody (ab226956) at 1/500 dilution + Human liver tissue extract at 30 µg12% SDS-PAGE gel.
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ELISA - Anti-EPO antibody (ab226956)An ELISA plate is coated with 50 μL of human EPO recombinant protein at concentration ranged from 0.125 μg/mL to 4 μg/mL. The coated protein is detected with EPO antibody at 1 μg/mL.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab226956 has not yet been referenced specifically in any publications.
