Anti-EPO antibody (ab226956)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-EPO antibody
See all EPO primary antibodies -
Description
Rabbit polyclonal to EPO -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment within Human EPO. The exact sequence is proprietary. NP_000790.
Database link: P01588 -
Positive control
- IHC-P: Rat liver tissue. WB: Human liver tissue extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab226956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/20000. |
Target
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Relevance
Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 2056 Human
- Entrez Gene: 24335 Rat
- Omim: 133170 Human
- SwissProt: P01588 Human
- SwissProt: P29676 Rat
- Unigene: 2303 Human
- Unigene: 11365 Rat
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Alternative names
- EP antibody
- EPO alpha antibody
- EPO antibody
see all
Images
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Western blot - Anti-EPO antibody (ab226956)Anti-EPO antibody (ab226956) at 1/500 dilution + Human liver tissue extract at 30 µg12% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO antibody (ab226956)Paraffin-embedded rat liver tissue stained for EPO using ab226956 at 1/400 dilution in immunohistochemical analysis.
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Western blot - Anti-EPO antibody (ab226956)Anti-EPO antibody (ab226956) at 1/10000 dilution + recombinant human EPO protein (with glycosylation) expressed in CHO cells at 0.1 µg12% SDS-PAGE gel.
References
ab226956 has not yet been referenced specifically in any publications.
