Key features and details
- Rabbit polyclonal to EPO
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Rat, Human, Recombinant fragment
- Isotype: IgG
Product nameAnti-EPO antibody
See all EPO primary antibodies
DescriptionRabbit polyclonal to EPO
Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
Species reactivityReacts with: Rat, Human, Recombinant fragment
Recombinant fragment within Human EPO (internal sequence). The exact sequence is proprietary.
Database link: P01588
- IHC-P: Rat liver tissue. WB: Human liver tissue extract.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/5000 - 1/20000.|
|ELISA||1/1000 - 1/10000.|
RelevanceHuman erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
- EP antibody
- EPO alpha antibody
- EPO antibody
Anti-EPO antibody (ab226956) at 1/10000 dilution + recombinant human EPO protein (with glycosylation) expressed in CHO cells at 0.1 µg
12% SDS-PAGE gel.
Paraffin-embedded rat liver tissue stained for EPO using ab226956 at 1/400 dilution in immunohistochemical analysis.
Anti-EPO antibody (ab226956) at 1/500 dilution + Human liver tissue extract at 30 µg
12% SDS-PAGE gel.
An ELISA plate is coated with 50 μL of human EPO recombinant protein at concentration ranged from 0.125 μg/mL to 4 μg/mL. The coated protein is detected with EPO antibody at 1 μg/mL.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226956 has not yet been referenced specifically in any publications.