Product nameAnti-EPO antibody
See all EPO primary antibodies
DescriptionRabbit polyclonal to EPO
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat
Recombinant full length protein corresponding to Human EPO.
Database link: P01588
- IHC-P: Rat brain and liver tissue. WB: Recombinant human EPO protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab273070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/5000 - 1/20000. Predicted molecular weight: 21 kDa.|
RelevanceHuman erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
- EP antibody
- EPO alpha antibody
- EPO antibody
Paraffin-embedded rat liver tissue stained for EPO using ab273070 at 1/400 in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
All lanes : Anti-EPO antibody (ab273070) at 1/20000 dilution
Lane 1 : Recombinant human EPO protein, 20 ng
Lane 2 : Recombinant human EPO protein, 100 ng
Predicted band size: 21 kDa
Paraffin-embedded rat brain tissue stained for EPO using ab273070 at 1/400 in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab273070 has not yet been referenced specifically in any publications.