Overview

  • Product name

    Anti-EPO antibody [EPO/1367] - BSA and Azide free
    See all EPO primary antibodies
  • Description

    Mouse monoclonal [EPO/1367] to EPO - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: Protein Array, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human EPO aa 28-162. The exact sequence is proprietary.
    Database link: P01588

  • Positive control

    • IHC-P: Human renal cell carcinoma and colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab224507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array Use at an assay dependent concentration.
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • EP antibody
    • EPO alpha antibody
    • EPO antibody
    • Epoetin antibody
    • Erythropoetin antibody
    • Erythropoietin precursor antibody
    • MVCD2 antibody
    see all

Images

  • This data was produced with ab224506, the same antibody in a different formulation with BSA and Azide.
    ab224506 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
  • Paraffin-embedded human renal cell carcinoma tissue stained for EPO using ab224507 (4 μg/ml) in immunohistochemical analysis.

  • Paraffin-embedded human colon carcinoma tissue stained for EPO using ab224507 (4 μg/ml) in immunohistochemical analysis.

References

ab224507 has not yet been referenced specifically in any publications.

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