Recombinant
RabMAb

Recombinant Anti-EPO-R antibody [EPR22366-40] (ab244202)

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Overview

  • Product name

    Anti-EPO-R antibody [EPR22366-40]
    See all EPO-R primary antibodies

  • Description

    Rabbit monoclonal [EPR22366-40] to EPO-R

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt,IHC-Fr,IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Mouse EPO-R aa 1-250. The exact sequence is proprietary.
    Database link: P14753

  • Positive control

    • IHC-P: Mouse E14.5 liver tissue. Mouse and rat spleen tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab244202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-Fr,IP or WB.

    • Target

    • Function

      Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.
      Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.

    • Tissue specificity

      Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells.

    • Involvement in disease

      Defects in EPOR are the cause of erythrocytosis familial type 1 (ECYT1) [MIM:133100]. ECYT1 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin and hematocrit, hypersensitivity of erythroid progenitors to erythropoietin, erythropoietin low serum levels, and no increase in platelets nor leukocytes. It has a relatively benign course and does not progress to leukemia.

    • Sequence similarities

      Belongs to the type I cytokine receptor family. Type 1 subfamily.
      Contains 1 fibronectin type-III domain.

    • Domain

      The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
      The box 1 motif is required for JAK interaction and/or activation.
      Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

    • Post-translational
      modifications

      On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site.
      Ubiquitinated by NOSIP; appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells.

    • Cellular localization

      Cell membrane and Secreted. Secreted and located to the cell surface.
    • Information by UniProt

    • Database links

      • Alternative names

        • EPO R antibody
        • EPO Receptor antibody
        • EPO-R antibody
        • epor antibody
        • EPOR_HUMAN antibody
        • Erythropoietin receptor antibody
        • Erythropoietin receptor precursor antibody
        • MGC138358 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)

        Immunohistochemical analysis of paraffin-embedded mouse E14.5 liver tissue labeling EPO-R with ab244202 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse E14.5 liver (PMID: 9808074) is observed. The section was incubated with ab244202 for 30mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

        Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)

        Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling EPO-R with ab244202 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on red pulp of the mouse spleen (PMID: 24283209) is observed. The section was incubated with ab244202 for 30mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

        Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)

        Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling EPO-R with ab244202 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on red pulp of the rat spleen (PMID: 24283209) is observed. The section was incubated with ab244202 for 30mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. 

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)

        Negative Control:

        Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling EPO-R with ab244202 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). No staining on mouse liver (PMID: 15259024) is observed. The section was incubated with ab244202 for 30mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

        Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

      References

      ab244202 has not yet been referenced specifically in any publications.

      Publishing research using ab244202? Please let us know so that we can cite the reference in this datasheet.

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