For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    epo-r-antibody-epr22366-40-ab244202.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Immunoglobulins Receptors
Share by email
RecombinantRabMAb

Recombinant Anti-EPO-R antibody [EPR22366-40] (ab244202)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)
  • Anti-EPO-R antibody [EPR22366-40] (ab244202)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22366-40] to EPO-R
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
Product image
Recombinant Human EPO-R protein (ab211321)

View more associated products

Overview

  • Product name

    Anti-EPO-R antibody [EPR22366-40]
    See all EPO-R primary antibodies
  • Description

    Rabbit monoclonal [EPR22366-40] to EPO-R
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse E14.5 liver tissue. Mouse and rat spleen tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22366-40
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Receptors
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Other
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cardiovascular
    • Atherosclerosis
    • Ischemia / Reperfusion
    • Cardiovascular
    • Heart
    • Apoptosis
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Alternative Versions

    • Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)
  • Recombinant Protein

    • Recombinant Human EPO-R protein (ab211321)
  • Related Products

    • Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab244202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Mouse
Rat
Application Abreviews Notes
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-Fr,IP or WB.
  • Target

    • Function

      Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.
      Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.
    • Tissue specificity

      Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells.
    • Involvement in disease

      Defects in EPOR are the cause of erythrocytosis familial type 1 (ECYT1) [MIM:133100]. ECYT1 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin and hematocrit, hypersensitivity of erythroid progenitors to erythropoietin, erythropoietin low serum levels, and no increase in platelets nor leukocytes. It has a relatively benign course and does not progress to leukemia.
    • Sequence similarities

      Belongs to the type I cytokine receptor family. Type 1 subfamily.
      Contains 1 fibronectin type-III domain.
    • Domain

      The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
      The box 1 motif is required for JAK interaction and/or activation.
      Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • Post-translational
      modifications

      On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site.
      Ubiquitinated by NOSIP; appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells.
    • Cellular localization

      Cell membrane and Secreted. Secreted and located to the cell surface.
    • Target information above from: UniProt accession P19235 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 13857 Mouse
      • Entrez Gene: 24336 Rat
      • SwissProt: P14753 Mouse
      • SwissProt: Q07303 Rat
      • Unigene: 2653 Mouse
      • Unigene: 22394 Rat
      • Alternative names

        • EPO R antibody
        • EPO Receptor antibody
        • EPO-R antibody
        • epor antibody
        • EPOR_HUMAN antibody
        • Erythropoietin receptor antibody
        • Erythropoietin receptor precursor antibody
        • MGC138358 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)

        Immunohistochemical analysis of paraffin-embedded mouse E14.5 liver tissue labeling EPO-R with ab244202 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse E14.5 liver (PMID: 9808074) is observed. The section was incubated with ab244202 for 30mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

        Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)

        Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling EPO-R with ab244202 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on red pulp of the mouse spleen (PMID: 24283209) is observed. The section was incubated with ab244202 for 30mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

        Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)

        Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling EPO-R with ab244202 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on red pulp of the rat spleen (PMID: 24283209) is observed. The section was incubated with ab244202 for 30mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. 

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] (ab244202)

        Negative Control:

        Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling EPO-R with ab244202 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). No staining on mouse liver (PMID: 15259024) is observed. The section was incubated with ab244202 for 30mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

        Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

      • Anti-EPO-R antibody [EPR22366-40] (ab244202)
        Anti-EPO-R antibody [EPR22366-40] (ab244202)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab244202? Please let us know so that we can cite the reference in this datasheet.

      ab244202 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab244202.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.