Key features and details
- Rabbit polyclonal to Epoxide hydrolase
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Epoxide hydrolase antibody
See all Epoxide hydrolase primary antibodies
DescriptionRabbit polyclonal to Epoxide hydrolase
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig
Recombinant protein fragment corresponding to a region within amino acids 64 and 262 of human Epoxide hydrolase (NP_000111)
- 293T, H1299 and HeLa cells. SG xenograft.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 53 kDa.|
|IHC-P||1/100 - 1/500.|
|ICC/IF||1/100 - 1/200.|
FunctionBiotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water.
Tissue specificityFound in liver.
Involvement in diseaseNote=In some populations, the high activity haplotype tyr113/his139 is overrepresented among women suffering from pregnancy-induced hypertension (pre-eclampsia) when compared with healthy controls.
Defects in EPHX1 are a cause of familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.
Sequence similaritiesBelongs to the peptidase S33 family.
Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
- Information by UniProt
- EPHX 1 antibody
- EPHX antibody
- Ephx1 antibody
All lanes : Anti-Epoxide hydrolase antibody (ab96774) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa
Gel conc 10% (SDS PAGE)
Immunohistochemical analysis of paraffin-embedded SG xenograft, using ab96774 at 1/100 dilution.
Immunofluorescence analysis of methanol fixed HeLa, using ab96774 at 1/200 dilution (top image) and costained with Hoescht 33342 (bottom image).
ab96774 has been referenced in 1 publication.
- Ortiz C et al. Cytotoxicity and Genotoxicity Assessment of Sandalwood Essential Oil in Human Breast Cell Lines MCF-7 and MCF-10A. Evid Based Complement Alternat Med 2016:3696232 (2016). WB . PubMed: 27293457