Overview

  • Product name

  • Description

    Rabbit polyclonal to EPS15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human EPS15 aa 798-874.
    Sequence:

    DPFKLNDPFQPFPGNDSPKEKDPEIFCDPFTSATTTTNKEADPSNFANFS AYPSEEDMIEWAKRESEREEEQRLARL


    Database link: P42566

  • Positive control

    • ICC/IF: U-2 OS cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab244519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Relevance

    The eps15 protein (EGFR pathway substrate clone 15) is tyrosine phosphorylated by the activation of the EGF and PDGF receptors. It was first identified as an 896 amino acid protein in mouse. Subsequent cloning of the human protein showed 90% homology to the mouse form. Several physiological functions have been proposed for eps15. Phosphorylation by EGF and PDGF receptors indicates that eps15 plays a role in signal transduction, cell proliferation and endocytosis.
  • Cellular localization

    Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Recruited to the plasma membrane upon EGFR activation and localizes to coated pits.
  • Database links

  • Alternative names

    • AF 1P antibody
    • AF 1p protein antibody
    • AF1P antibody
    • ALL1 fused gene from chromosome 1 antibody
    • Epidermal growth factor receptor pathway substrate 15 antibody
    • Epidermal growth factor receptor substrate 15 antibody
    • EPS 15 antibody
    • MLLT 5 antibody
    • MLLT5 antibody
    • Protein Eps 15 antibody
    • Protein Eps15 antibody
    see all

Images

  • PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for EPS15 (green) using ab244519 at 4 µg/ml in ICC/IF.

References

ab244519 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab244519.
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