Overview

  • Product name

  • Description

    Rabbit polyclonal to EPS15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 575 and 625 of human EPS15 (NP_001972.1).

  • Positive control

    • Human Breast Carcinoma, Colon Carcinoma, Linitis Plastica Stomach Cancer, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma and Testicular Seminoma tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      The eps15 protein (EGFR pathway substrate clone 15) is tyrosine phosphorylated by the activation of the EGF and PDGF receptors. It was first identified as an 896 amino acid protein in mouse. Subsequent cloning of the human protein showed 90% homology to the mouse form. Several physiological functions have been proposed for eps15. Phosphorylation by EGF and PDGF receptors indicates that eps15 plays a role in signal transduction, cell proliferation and endocytosis.
    • Cellular localization

      Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Recruited to the plasma membrane upon EGFR activation and localizes to coated pits.
    • Database links

    • Alternative names

      • AF 1P antibody
      • AF 1p protein antibody
      • AF1P antibody
      • ALL1 fused gene from chromosome 1 antibody
      • Epidermal growth factor receptor pathway substrate 15 antibody
      • Epidermal growth factor receptor substrate 15 antibody
      • EPS 15 antibody
      • MLLT 5 antibody
      • MLLT5 antibody
      • Protein Eps 15 antibody
      • Protein Eps15 antibody
      see all

    Images

    • ab84810, at 1/100 dilution, staining EPS15 in formalin-fixed, paraffin-embedded human testicular seminoma by Immunohistochemistry using a Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Hilyte PlusTM 555 secondary antibody at a dilution of 1/100.
    • ab84810, at 1/500 dilution, staining EPS15 in formalin-fixed, paraffin-embedded human testicular seminoma by Immunohistochemistry using DAB staining.
    • ab84810, at 1/500 dilution, staining EPS15 in formalin-fixed, paraffin-embedded human prostate carcinoma by Immunohistochemistry using DAB staining.

    References

    ab84810 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab84810.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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