Product nameAnti-EPS15 antibody [EP9280]
See all EPS15 primary antibodies
DescriptionRabbit monoclonal [EP9280] to EPS15
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human EPS15 aa 800 to the C-terminus. The exact sequence is proprietary.
Database link: P42566
- A431and Jurkat cell lysates. Human fetal brain tissue lysate. Human breast tissue. Human thyroid gland carcinoma tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 99 kDa.|
|ICC/IF||1/100 - 1/250.|
RelevanceThe eps15 protein (EGFR pathway substrate clone 15) is tyrosine phosphorylated by the activation of the EGF and PDGF receptors. It was first identified as an 896 amino acid protein in mouse. Subsequent cloning of the human protein showed 90% homology to the mouse form. Several physiological functions have been proposed for eps15. Phosphorylation by EGF and PDGF receptors indicates that eps15 plays a role in signal transduction, cell proliferation and endocytosis.
Cellular localizationCytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Recruited to the plasma membrane upon EGFR activation and localizes to coated pits.
- AF 1P antibody
- AF 1p protein antibody
- AF1P antibody
All lanes : Anti-EPS15 antibody [EP9280] (ab174291) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Human fetal brain tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 99 kDa
Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling EPS15 using ab174289 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling EPS15 using ab174289 at 1/50 dilution.
ab174291 has not yet been referenced specifically in any publications.