Overview

  • Product name
    Anti-EPS15 antibody [EP9280]
    See all EPS15 primary antibodies
  • Description
    Rabbit monoclonal [EP9280] to EPS15
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human EPS15 aa 800 to the C-terminus. The exact sequence is proprietary.
    Database link: P42566

  • Positive control
    • A431and Jurkat cell lysates. Human fetal brain tissue lysate. Human breast tissue. Human thyroid gland carcinoma tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EP9280
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 99 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Relevance
      The eps15 protein (EGFR pathway substrate clone 15) is tyrosine phosphorylated by the activation of the EGF and PDGF receptors. It was first identified as an 896 amino acid protein in mouse. Subsequent cloning of the human protein showed 90% homology to the mouse form. Several physiological functions have been proposed for eps15. Phosphorylation by EGF and PDGF receptors indicates that eps15 plays a role in signal transduction, cell proliferation and endocytosis.
    • Cellular localization
      Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Recruited to the plasma membrane upon EGFR activation and localizes to coated pits.
    • Database links
    • Alternative names
      • AF 1P antibody
      • AF 1p protein antibody
      • AF1P antibody
      • ALL1 fused gene from chromosome 1 antibody
      • Epidermal growth factor receptor pathway substrate 15 antibody
      • Epidermal growth factor receptor substrate 15 antibody
      • EPS 15 antibody
      • MLLT 5 antibody
      • MLLT5 antibody
      • Protein Eps 15 antibody
      • Protein Eps15 antibody
      see all

    Images

    • All lanes : Anti-EPS15 antibody [EP9280] (ab174291) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Human fetal brain tissue lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 99 kDa

    • Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling EPS15 using ab174289 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling EPS15 using ab174289 at 1/50 dilution.

    References

    ab174291 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab174291.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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