Overview

  • Product name

    Anti-EPS15R antibody [EP1146Y]
    See all EPS15R primary antibodies
  • Description

    Rabbit monoclonal [EP1146Y] to EPS15R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human EPS15R aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q9UBC2

  • Positive control

    • HeLa, 293T and SHSY5Y cell lysates; A431 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab76004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/20000. Predicted molecular weight: 94 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Seems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.
    • Sequence similarities

      Contains 1 EF-hand domain.
      Contains 3 EH domains.
    • Post-translational
      modifications

      Phosphorylated on tyrosine residues by EGFR.
    • Cellular localization

      Cell membrane. Nucleus. Membrane > coated pit. Localized to plasma membrane coated pits.
    • Information by UniProt
    • Database links

    • Alternative names

      • EP15R_HUMAN antibody
      • epidermal growth factor receptor pathway substrate 15- ike 1 antibody
      • Epidermal growth factor receptor substrate 15 like 1 antibody
      • Epidermal growth factor receptor substrate 15-like 1 antibody
      • epidermal growth factor receptor substrate EPS15R antibody
      • Eps15 related protein antibody
      • Eps15-related protein antibody
      • EPS15L1 antibody
      • Eps15R antibody
      see all

    Images

    • All lanes : Anti-EPS15R antibody [EP1146Y] (ab76004) at 1/20000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : 293T cell lysates
      Lane 3 : SHSY5Y cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 94 kDa
      Observed band size: 108,125,76 kDa
      why is the actual band size different from the predicted?



      There are three isoforms with MW at 125, 108 and 76 kDa respectively.

    References

    This product has been referenced in:

    • Fukushima T  et al. Nedd4-induced monoubiquitination of IRS-2 enhances IGF signalling and mitogenic activity. Nat Commun 6:6780 (2015). Read more (PubMed: 25879670) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab76004.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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