Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1146Y] to EPS15R - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-EPS15R antibody [EP1146Y] - BSA and Azide free
See all EPS15R primary antibodies
DescriptionRabbit monoclonal [EP1146Y] to EPS15R - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human EPS15R aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9UBC2
ab247425 is the carrier-free version of ab76004. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247425 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
Our Abpromise guarantee covers the use of ab247425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 94 kDa.|
FunctionSeems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.
Sequence similaritiesContains 1 EF-hand domain.
Contains 3 EH domains.
modificationsPhosphorylated on tyrosine residues by EGFR.
Cellular localizationCell membrane. Nucleus. Membrane > coated pit. Localized to plasma membrane coated pits.
- Information by UniProt
- EP15R_HUMAN antibody
- epidermal growth factor receptor pathway substrate 15- ike 1 antibody
- Epidermal growth factor receptor substrate 15 like 1 antibody
All lanes : Anti-EPS15R antibody [EP1146Y] (ab76004) at 1/20000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : 293T cell lysates
Lane 3 : SHSY5Y cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 94 kDa
Observed band size: 108,125,76 kDa why is the actual band size different from the predicted?
This data was developed using ab76004, the same antibody clone in a different buffer formulation.
There are three isoforms with MW at 125, 108 and 76 kDa respectively.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab247425 has not yet been referenced specifically in any publications.