Overview

  • Product name

    Anti-EPS15R antibody [EP1147Y]
    See all EPS15R primary antibodies
  • Description

    Rabbit monoclonal [EP1147Y] to EPS15R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human EPS15R aa 800-900 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa cell membrane or nuclear cell lysate Human breast carcinoma tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab53006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Detects a band of approximately 125 kDa (predicted molecular weight: 94 kDa).
IP 1/70.
ICC 1/50 - 1/100.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Seems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.
  • Sequence similarities

    Contains 1 EF-hand domain.
    Contains 3 EH domains.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues by EGFR.
  • Cellular localization

    Cell membrane. Nucleus. Membrane > coated pit. Localized to plasma membrane coated pits.
  • Information by UniProt
  • Database links

  • Alternative names

    • EP15R_HUMAN antibody
    • epidermal growth factor receptor pathway substrate 15- ike 1 antibody
    • Epidermal growth factor receptor substrate 15 like 1 antibody
    • Epidermal growth factor receptor substrate 15-like 1 antibody
    • epidermal growth factor receptor substrate EPS15R antibody
    • Eps15 related protein antibody
    • Eps15-related protein antibody
    • EPS15L1 antibody
    • Eps15R antibody
    see all

Images

  • Anti-EPS15R antibody [EP1147Y] (ab53006) at 1/50000 dilution + HeLa cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP labelled at 1/2000 dilution

    Predicted band size: 94 kDa
    Observed band size: 125 kDa
    why is the actual band size different from the predicted?

  • ab53006, at a 1/50 dilution, staining Human EPS15R in Breast Carcinoma, using Immunohistochemsitry, Paraffin embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

References

This product has been referenced in:

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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