Product nameAnti-EPS15R antibody [EP1147Y]
See all EPS15R primary antibodies
DescriptionRabbit monoclonal [EP1147Y] to EPS15R
Tested applicationsSuitable for: WB, IP, ICC, Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human EPS15R aa 800-900 (C terminal). The exact sequence is proprietary.
- HeLa cell membrane or nuclear cell lysate Human breast carcinoma tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab53006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000. Detects a band of approximately 125 kDa (predicted molecular weight: 94 kDa).|
|ICC||1/50 - 1/100.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionSeems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.
Sequence similaritiesContains 1 EF-hand domain.
Contains 3 EH domains.
modificationsPhosphorylated on tyrosine residues by EGFR.
Cellular localizationCell membrane. Nucleus. Membrane > coated pit. Localized to plasma membrane coated pits.
- Information by UniProt
- EP15R_HUMAN antibody
- epidermal growth factor receptor pathway substrate 15- ike 1 antibody
- Epidermal growth factor receptor substrate 15 like 1 antibody
Anti-EPS15R antibody [EP1147Y] (ab53006) at 1/50000 dilution + HeLa cell lysate at 10 µg
goat anti-rabbit HRP labelled at 1/2000 dilution
Predicted band size: 94 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
ab53006, at a 1/50 dilution, staining Human EPS15R in Breast Carcinoma, using Immunohistochemsitry, Paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This product has been referenced in:
- Evergren E et al. Eps15R and clathrin regulate EphB2-mediated cell repulsion. Traffic 19:44-57 (2018). Read more (PubMed: 28972287) »
- Liu Z & Zheng Y A requirement for epsin in mitotic membrane and spindle organization. J Cell Biol 186:473-80 (2009). Read more (PubMed: 19704019) »