Overview

  • Product name

  • Description

    Rabbit polyclonal to EPS8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 761- 822 (DELRTVCPEG ARVYSQITVQ KAALEDSSGS SELQEIMRRR QEKISAAASD SGVESFDEGS SH) of Human EPS8 (NP_004438).

  • Positive control

    • WB: H1299, HeLa cell lysates IHC-P: Gastric CA

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified by antigen affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 92 kDa.
IHC-P 1/100 - 1/250.

Target

  • Function

    Upon binding to EGF receptor enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets.
  • Tissue specificity

    Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells.
  • Sequence similarities

    Belongs to the EPS8 family.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • Domain

    The SH3 domain mediates interaction with SHB.
  • Post-translational
    modifications

    Phosphorylated by several receptor tyrosine kinases.
  • Information by UniProt
  • Database links

  • Alternative names

    • Epidermal growth factor receptor kinase substrate 8 antibody
    • Epidermal growth factor receptor pathway substrate 8 antibody
    • EPS 8 antibody
    • EPS8 antibody
    • EPS8_HUMAN antibody
    see all

Images

  • All lanes : Anti-EPS8 antibody (ab96144) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : Hela whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 92 kDa



    7.5% SDS PAGE
  • ab96144, at 1/100 dilution, staining EPS8 in paraffin embedded Gastric CA by Immunohistochemical analysis.

References

This product has been referenced in:

  • Huang R  et al. EPS8 regulates proliferation, apoptosis and chemosensitivity in BCR-ABL positive cells via the BCR-ABL/PI3K/AKT/mTOR pathway. Oncol Rep 39:119-128 (2018). Read more (PubMed: 29192326) »
  • Mok KW  et al. Rictor/mTORC2 regulates blood-testis barrier dynamics via its effects on gap junction communications and actin filament network. FASEB J 27:1137-52 (2013). Read more (PubMed: 23288930) »
See all 3 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab96144.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up