Overview

  • Product name

    Anti-EPS8 antibody [EPR6112]
    See all EPS8 primary antibodies
  • Description

    Rabbit monoclonal [EPR6112] to EPS8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human EPS8 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, A375, 293T, and K562 cell lysates, Human kidney tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6112
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 92 kDa).
IHC-P 1/100 - 1/250. antigen retrieval is recommended.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Upon binding to EGF receptor enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets.
    • Tissue specificity

      Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells.
    • Sequence similarities

      Belongs to the EPS8 family.
      Contains 1 PH domain.
      Contains 1 SH3 domain.
    • Domain

      The SH3 domain mediates interaction with SHB.
    • Post-translational
      modifications

      Phosphorylated by several receptor tyrosine kinases.
    • Information by UniProt
    • Database links

    • Alternative names

      • Epidermal growth factor receptor kinase substrate 8 antibody
      • Epidermal growth factor receptor pathway substrate 8 antibody
      • EPS 8 antibody
      • EPS8 antibody
      • EPS8_HUMAN antibody
      see all

    Images

    • All lanes : Anti-EPS8 antibody [EPR6112] (ab124882) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A375 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 92 kDa

    • Staining of EPS8 in Paraffin-embedded Human kidney tissue by Immunohistochemistry using ab124882 at a dilution of 1/100.

    References

    This product has been referenced in:

    • Lu H  et al. Identification of HLA-A*1101-restricted cytotoxic T lymphocyte epitopes derived from epidermal growth factor pathway substrate number 8. Mol Med Rep 14:4999-5006 (2016). WB ; Human . Read more (PubMed: 27840923) »
    • Tang B  et al. Identification of human leukemia antigen A*0201-restricted epitopes derived from epidermal growth factor pathway substrate number 8. Mol Med Rep 12:1741-52 (2015). Read more (PubMed: 25936538) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab124882.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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