Key features and details
- Rabbit polyclonal to EPS8L1
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EPS8L1 antibody
See all EPS8L1 primary antibodies
DescriptionRabbit polyclonal to EPS8L1
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Dog
- WB: HepG2 cell lysate. IHC-P: Human placental tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab58687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1:1562500.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 80 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
FunctionStimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton.
Tissue specificityDetected in placenta.
Sequence similaritiesBelongs to the EPS8 family.
Contains 1 SH3 domain.
- Information by UniProt
- DRC3 antibody
- Epidermal growth factor receptor kinase substrate 8 like protein 1 antibody
- Epidermal growth factor receptor kinase substrate 8-like protein 1 antibody
Anti-EPS8L1 antibody (ab58687) at 1.25 µg/ml + Hep2 cell lysate at 10µg.
HRP conjugated anti-Rabbit IgG at 1/50000 dilution.
Predicted band size: 80 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded) analysis of human placenta tissue labelling EPS8L1 with ab58687 at 4-8µg/ml. Arrows indicate positively labelled decidual cells. Magnification: 400X.
ab58687 has not yet been referenced specifically in any publications.