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    eps8l2-antibody-ab126155.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins
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Anti-EPS8L2 antibody (ab126155)

  • Datasheet
  • SDS
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Western blot - Anti-EPS8L2 antibody (ab126155)

    Key features and details

    • Rabbit polyclonal to EPS8L2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human EPS8L2 protein (ab132633)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-EPS8L2 antibody
      See all EPS8L2 primary antibodies
    • Description

      Rabbit polyclonal to EPS8L2
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant fragment containing a sequence corresponding to a region within amino acids 402-659 of Human EPS8L2. (Uniprot: Q9H6S3)

    • Positive control

      • A431 whole cell lysate and HepG2 whole cell lysate
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Actin Binding Proteins
      • Signal Transduction
      • Growth Factors/Hormones
      • EGF

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • A431 whole cell lysate (ab7909)
    • Recombinant Protein

      • Recombinant Human EPS8L2 protein (ab132633)

    Applications

    Our Abpromise guarantee covers the use of ab126155 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/3000. Predicted molecular weight: 81 kDa.

    Target

    • Function

      Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton.
    • Tissue specificity

      Detected in fibroblasts and placenta.
    • Sequence similarities

      Belongs to the EPS8 family.
      Contains 1 PID domain.
      Contains 1 SH3 domain.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession Q9H6S3 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 64787 Human
      • Omim: 614988 Human
      • SwissProt: Q9H6S3 Human
      • Unigene: 55016 Human
      • Alternative names

        • Epidermal growth factor receptor kinase substrate 8 like protein 2 antibody
        • Epidermal growth factor receptor kinase substrate 8-like protein 2 antibody
        • Epidermal growth factor receptor pathway substrate 8 like protein 2 antibody
        • Epidermal growth factor receptor pathway substrate 8 related protein 2 antibody
        • Epidermal growth factor receptor pathway substrate 8-related protein 2 antibody
        • EPS8 like 2 antibody
        • EPS8 like protein 2 antibody
        • EPS8 related protein 2 antibody
        • EPS8-like protein 2 antibody
        • EPS8-related protein 2 antibody
        • EPS8L 2 antibody
        • EPS8L2 antibody
        • EPS8R 2 antibody
        • EPS8R2 antibody
        • ES8L2_HUMAN antibody
        • FLJ16738 antibody
        • FLJ21935 antibody
        • FLJ22171 antibody
        • MGC126530 antibody
        • MGC3088 antibody
        see all

      Images

      • Western blot - Anti-EPS8L2 antibody (ab126155)
        Western blot - Anti-EPS8L2 antibody (ab126155)
        All lanes : Anti-EPS8L2 antibody (ab126155) at 1/1000 dilution

        Lane 1 : A431 whole cell lysate
        Lane 2 : HepG2 whole cell lysate

        Lysates/proteins at 30 µg per lane.

        Predicted band size: 81 kDa



        7.5% SDS PAGE

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab126155? Please let us know so that we can cite the reference in this datasheet.

      ab126155 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Western blot abreview for Anti-EPS8L2 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell (Brain)
      Gel Running Conditions
      Reduced Non-Denaturing (Native) (4-20 gradient)
      Loading amount
      40 µg
      Specification
      Brain
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 27 2019

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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