Key features and details
- Rabbit polyclonal to EPS8L2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EPS8L2 antibody
See all EPS8L2 primary antibodies
DescriptionRabbit polyclonal to EPS8L2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
A region between residue 600 and 650 of human EPS8L2 (NP_073609.2)
- HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab85960 was affinity purified using an epitope specific to EPS8L2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab85960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionStimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton.
Tissue specificityDetected in fibroblasts and placenta.
Sequence similaritiesBelongs to the EPS8 family.
Contains 1 PID domain.
Contains 1 SH3 domain.
- Information by UniProt
- Epidermal growth factor receptor kinase substrate 8 like protein 2 antibody
- Epidermal growth factor receptor kinase substrate 8-like protein 2 antibody
- Epidermal growth factor receptor pathway substrate 8 like protein 2 antibody
All lanes : Anti-EPS8L2 antibody (ab85960) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 81 kDa
Observed band size: 81 kDa
Exposure time: 30 seconds
Detection of Human EPS8L2 by Immunoprecipitation, using ab85960 at 3µg/mg lysate. Image shows immunoprecipitated EPS8L2 detected post IP by WB, loading 20% of IP using HeLa whole cell lysate at 1mg, with ab85960 at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
ab85960 has not yet been referenced specifically in any publications.