Overview

  • Product name

    Anti-Epsin 1 antibody [6F7F9]
    See all Epsin 1 primary antibodies
  • Description

    Mouse monoclonal [6F7F9] to Epsin 1
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Epsin 1 aa 106-254. Expressed in E coli.
    Sequence:

    LKDFQ YVDRDGKDQG VNVREKAKQL VALLRDEDRL REERAHALKT KEKLAQTATA SSAAVGSGPP PEAEQAWPQS SGEEELQLQL ALAMSKEEAD QPPSCGPEDD AQLQLALSLS REEHDKEERI RRGDDLRLQM AIEESKRETG GKEE


    Database link: Q9Y6I3

  • Positive control

    • Recombinant protein, Epsin 1 transfected HEK283 cell lysate, A431 cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    6F7F9
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab204952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200 - 1/400.
WB 1/500 - 1/2000. Predicted molecular weight: 60 kDa.

Target

  • Function

    Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.
  • Sequence similarities

    Belongs to the epsin family.
    Contains 1 ENTH (epsin N-terminal homology) domain.
    Contains 3 UIM (ubiquitin-interacting motif) repeats.
  • Domain

    The NPF repeat domain is involved in EPS15 binding.
    The DPW repeat domain is involved in AP2A2 and clathrin binding.
    The [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction with the AP-2 complex subunit AP2B1.
  • Post-translational
    modifications

    Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation.
    Ubiquitinated.
  • Cellular localization

    Cytoplasm. Cell membrane. Nucleus. Membrane > clathrin-coated pit. Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145.
  • Information by UniProt
  • Database links

  • Alternative names

    • EH domain binding mitotic phosphoprotein antibody
    • EH domain-binding mitotic phosphoprotein antibody
    • EPN 1 antibody
    • Epn1 antibody
    • EPN1_HUMAN antibody
    • EPS 15 interacting protein 1 antibody
    • EPS-15-interacting protein 1 antibody
    • Epsin 1 antibody
    • Epsin-1 antibody
    see all

Images

  • Anti-Epsin 1 antibody [6F7F9] (ab204952) at 1/500 dilution + Recombinant Epsin 1 (aa 106-254) protein

    Predicted band size: 60 kDa



    (Expected MW is 42.4 kDa)

  • All lanes : Anti-Epsin 1 antibody [6F7F9] (ab204952) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Epsin 1 (106-254) - hIgGFc transfected HEK293 cell lysate

    Predicted band size: 60 kDa

  • Flow cytometric analysis of A431 cells labeling Epsin 1 with ab204952 at 1/200 dilution (green) or negative control (red)

References

ab204952 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab204952.
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