Overview

  • Product name

  • Description

    Rabbit polyclonal to Epsin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment (His-tag) corresponding to Human Epsin 1 aa 279-437. (Expressed in E.coli).
    Sequence:

    PAPMAAAVPTAAPTSDPWGGPPVPPAADPWGGPAPTPASGDPWRPAAPAG PSVDPWGGTPAPAAGEGPTPDPWGSSDGGVPVSGPSASDPWTPAPAFSDP WGGSPAKPSTNGTTAAGGFDTEPDEFSDFDRLRTALPTSGSSAGELELLA GEVPARSPG


    Database link: Q9Y6I3

  • Positive control

    • WB: Recombinant human Epsin 1 protein; HeLa and HEK-293T cell lysates; Mouse cerebrum and heart lysates. IHC-P: Human cerebrum tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab232764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 60 kDa.
IHC-P Use a concentration of 5 - 20 µg/ml.

Target

  • Function

    Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.
  • Sequence similarities

    Belongs to the epsin family.
    Contains 1 ENTH (epsin N-terminal homology) domain.
    Contains 3 UIM (ubiquitin-interacting motif) repeats.
  • Domain

    The NPF repeat domain is involved in EPS15 binding.
    The DPW repeat domain is involved in AP2A2 and clathrin binding.
    The [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction with the AP-2 complex subunit AP2B1.
  • Post-translational
    modifications

    Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation.
    Ubiquitinated.
  • Cellular localization

    Cytoplasm. Cell membrane. Nucleus. Membrane > clathrin-coated pit. Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145.
  • Information by UniProt
  • Database links

  • Alternative names

    • EH domain binding mitotic phosphoprotein antibody
    • EH domain-binding mitotic phosphoprotein antibody
    • EPN 1 antibody
    • Epn1 antibody
    • EPN1_HUMAN antibody
    • EPS 15 interacting protein 1 antibody
    • EPS-15-interacting protein 1 antibody
    • Epsin 1 antibody
    • Epsin-1 antibody
    see all

Images

  • Anti-Epsin 1 antibody (ab232764) at 2 µg/ml + Recombinant human Epsin 1 protein

    Predicted band size: 60 kDa

  • Anti-Epsin 1 antibody (ab232764) at 2 µg + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Predicted band size: 60 kDa

  • Anti-Epsin 1 antibody (ab232764) at 2 µg + Mouse cerebrum lysate

    Predicted band size: 60 kDa

  • Anti-Epsin 1 antibody (ab232764) at 2 µg/ml + Mouse heart lysate

    Predicted band size: 60 kDa

  • Anti-Epsin 1 antibody (ab232764) at 2 µg/ml + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

    Predicted band size: 60 kDa

  • Formalin-fixed, paraffin-embedded human cerebrum tissue stained for Epsin 1 using ab232764 at 20 μg/ml in immunohistochemical analysis. DAB staining.

References

ab232764 has not yet been referenced specifically in any publications.

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