Recombinant
RabMAb

Recombinant Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)

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Overview

  • Product name

    Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free
    See all Epsin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3023] to Epsin 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Epsin 1 aa 450-550. The exact sequence is proprietary.
    Database link: Q9Y6I3

  • General notes

    Ab247415 is the carrier-free version of ab75879. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247415 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab247415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.

    • Sequence similarities

      Belongs to the epsin family.
      Contains 1 ENTH (epsin N-terminal homology) domain.
      Contains 3 UIM (ubiquitin-interacting motif) repeats.

    • Domain

      The NPF repeat domain is involved in EPS15 binding.
      The DPW repeat domain is involved in AP2A2 and clathrin binding.
      The [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction with the AP-2 complex subunit AP2B1.

    • Post-translational
      modifications

      Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation.
      Ubiquitinated.

    • Cellular localization

      Cytoplasm. Cell membrane. Nucleus. Membrane > clathrin-coated pit. Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • EH domain binding mitotic phosphoprotein antibody
      • EH domain-binding mitotic phosphoprotein antibody
      • EPN 1 antibody
      • Epn1 antibody
      • EPN1_HUMAN antibody
      • EPS 15 interacting protein 1 antibody
      • EPS-15-interacting protein 1 antibody
      • Epsin 1 antibody
      • Epsin-1 antibody
      see all

    References

    ab247415 has not yet been referenced specifically in any publications.

    Publishing research using ab247415? Please let us know so that we can cite the reference in this datasheet.

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