RecombinantRabMAb

Recombinant Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)

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Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
  • Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
  • Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
  • Immunocytochemistry - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
  • Immunocytochemistry - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
  • Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
  • Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3023] to Epsin 1 - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free
    See all Epsin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3023] to Epsin 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Epsin 1 aa 450-550. The exact sequence is proprietary.
    Database link: Q9Y6I3

  • General notes

    Ab247415 is the carrier-free version of ab75879. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247415 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab247415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.

    • Sequence similarities

      Belongs to the epsin family.
      Contains 1 ENTH (epsin N-terminal homology) domain.
      Contains 3 UIM (ubiquitin-interacting motif) repeats.

    • Domain

      The NPF repeat domain is involved in EPS15 binding.
      The DPW repeat domain is involved in AP2A2 and clathrin binding.
      The [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction with the AP-2 complex subunit AP2B1.

    • Post-translational
      modifications

      Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation.
      Ubiquitinated.

    • Cellular localization

      Cytoplasm. Cell membrane. Nucleus. Membrane > clathrin-coated pit. Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • EH domain binding mitotic phosphoprotein antibody
      • EH domain-binding mitotic phosphoprotein antibody
      • EPN 1 antibody
      • Epn1 antibody
      • EPN1_HUMAN antibody
      • EPS 15 interacting protein 1 antibody
      • EPS-15-interacting protein 1 antibody
      • Epsin 1 antibody
      • Epsin-1 antibody
      see all

    Images

    • Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
      Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
      Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/5000 dilution (purified) + rat brain at 20 µg

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

      Predicted band size: 58 kDa
      Observed band size: 94 kDa
      why is the actual band size different from the predicted?



      This data was developed using ab75879, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
      Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
      All lanes : Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/1000 dilution (purified)

      Lane 1 : 293T cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : U-87 MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

      Predicted band size: 58 kDa
      Observed band size: 94 kDa why is the actual band size different from the predicted?



      This data was developed using ab75879, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
      Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
      Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/5000 dilution (purified) + mouse brain tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

      Predicted band size: 58 kDa
      Observed band size: 94 kDa why is the actual band size different from the predicted?



      This data was developed using ab75879, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
      Immunocytochemistry - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
      This data was developed using ab75879, the same antibody clone in a different buffer formulation.Immunofluorescence staining of HeLa cells with purified ab75879 at a working dilution of 1/50, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab75879 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
    • Immunocytochemistry - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
      Immunocytochemistry - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
      This data was developed using ab75879, the same antibody clone in a different buffer formulation.Unpurified ab75879, at a 1/100 dilution, staining Epsin 1 in SH-SY5Y cells by Immunofluorescence.
    • Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
      Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
      All lanes : Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/2000 dilution (unpurified)

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit-HRP at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 58 kDa



      This data was developed using ab75879, the same antibody clone in a different buffer formulation.

       

    • Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
      Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab247415? Please let us know so that we can cite the reference in this datasheet.

    ab247415 has not yet been referenced specifically in any publications.

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