Overview

  • Product name

  • Description

    Rabbit polyclonal to Epsin 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Monkey, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human Epsin 2 (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: O95208

  • Positive control

    • IHC-P: Human brain cortex tissue. WB: HEK-293T and NIH/3T3 whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 30% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 68 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Plays a role in the formation of clathrin-coated invaginations and endocytosis.
  • Tissue specificity

    Highest expression is found in brain. Detected at lower levels in lung and liver.
  • Sequence similarities

    Belongs to the epsin family.
    Contains 1 ENTH (epsin N-terminal homology) domain.
    Contains 2 UIM (ubiquitin-interacting motif) repeats.
  • Domain

    The NPF repeat domain is involved in EPS15 binding.
    The DPW repeat domain is involved in AP-2 and clathrin binding.
  • Post-translational
    modifications

    Ubiquitinated.
  • Cellular localization

    Cytoplasm. Cytoplasmic vesicle > clathrin-coated vesicle. In punctate structures throughout the cell, associated with clathrin-coated vesicles, and particularly concentrated in the region of the Golgi complex.
  • Information by UniProt
  • Database links

  • Alternative names

    • EHB21 antibody
    • EPN 2 antibody
    • Epn2 antibody
    • EPN2_HUMAN antibody
    • EPS 15 interacting protein 2 antibody
    • EPS-15-interacting protein 2 antibody
    • Eps15 binding protein antibody
    • Epsin 2 antibody
    • Epsin-2 antibody
    • Epsin2 antibody
    • KIAA1065 antibody
    • OTTHUMP00000065808 antibody
    • OTTHUMP00000065809 antibody
    • OTTHUMP00000065810 antibody
    • OTTHUMP00000065811 antibody
    • OTTHUMP00000065889 antibody
    see all

Images

  • All lanes : Anti-Epsin 2 antibody (ab230546) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Developed using the ECL technique.

    Predicted band size: 68 kDa

  • Formalin-fixed, paraffin-embedded human brain cortex tissue stained for Epsin 2 with ab230546 at a 1:100 dilution in immunohistochemical analysis.

References

ab230546 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab230546.
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