Key features and details
- Rabbit polyclonal to Epsin 2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Epsin 2 antibody
See all Epsin 2 primary antibodies
DescriptionRabbit polyclonal to Epsin 2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Monkey, Zebrafish
Synthetic peptide corresponding to Human Epsin 2 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: O95208
- IHC-P: Human brain cortex tissue. WB: HEK-293T and NIH/3T3 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 68 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionPlays a role in the formation of clathrin-coated invaginations and endocytosis.
Tissue specificityHighest expression is found in brain. Detected at lower levels in lung and liver.
Sequence similaritiesBelongs to the epsin family.
Contains 1 ENTH (epsin N-terminal homology) domain.
Contains 2 UIM (ubiquitin-interacting motif) repeats.
DomainThe NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP-2 and clathrin binding.
Cellular localizationCytoplasm. Cytoplasmic vesicle > clathrin-coated vesicle. In punctate structures throughout the cell, associated with clathrin-coated vesicles, and particularly concentrated in the region of the Golgi complex.
- Information by UniProt
- EHB21 antibody
- EPN 2 antibody
- Epn2 antibody
All lanes : Anti-Epsin 2 antibody (ab230546) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Developed using the ECL technique.
Predicted band size: 68 kDa
Formalin-fixed, paraffin-embedded human brain cortex tissue stained for Epsin 2 with ab230546 at a 1:100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230546 has not yet been referenced specifically in any publications.