Anti-Epsin 2 antibody (ab242044)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Epsin 2 antibody
See all Epsin 2 primary antibodies -
Description
Rabbit polyclonal to Epsin 2 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human Epsin 2 aa 150-200. The exact sequence is proprietary.
Database link: O95208 -
Positive control
- IP: Epsin 2 IP in HeLa whole cell lysate.
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General notes
ab242044 has not performed satisfactorily when used for WB of Epsin 2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Epsin 2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to Epsin 2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab242044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Plays a role in the formation of clathrin-coated invaginations and endocytosis. -
Tissue specificity
Highest expression is found in brain. Detected at lower levels in lung and liver. -
Sequence similarities
Belongs to the epsin family.
Contains 1 ENTH (epsin N-terminal homology) domain.
Contains 2 UIM (ubiquitin-interacting motif) repeats. -
Domain
The NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP-2 and clathrin binding. -
Post-translational
modificationsUbiquitinated. -
Cellular localization
Cytoplasm. Cytoplasmic vesicle > clathrin-coated vesicle. In punctate structures throughout the cell, associated with clathrin-coated vesicles, and particularly concentrated in the region of the Golgi complex. - Information by UniProt
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Database links
- Entrez Gene: 22905 Human
- Entrez Gene: 13855 Mouse
- Omim: 607263 Human
- SwissProt: O95208 Human
- SwissProt: Q8CHU3 Mouse
- Unigene: 309467 Human
- Unigene: 139695 Mouse
- Unigene: 436466 Mouse
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Alternative names
- EHB21 antibody
- EPN 2 antibody
- Epn2 antibody
see all
Images
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Immunoprecipitation - Anti-Epsin 2 antibody (ab242044)Epsin 2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab242044 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab242044 at 0.4 µg/ml.
Lane 1: ab242044 IP in HeLa whole cell lysate.
lane 2: Control IgG in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 30 seconds.
Prepared using NETN lysis buffer.
Datasheets and documents
References
ab242044 has not yet been referenced specifically in any publications.
