Anti-Epsin 2 antibody (ab242044)
Key features and details
- Rabbit polyclonal to Epsin 2
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Epsin 2 antibody
See all Epsin 2 primary antibodies -
Description
Rabbit polyclonal to Epsin 2 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human Epsin 2 aa 150-200. The exact sequence is proprietary.
Database link: O95208 -
Positive control
- IP: Epsin 2 IP in HeLa whole cell lysate.
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General notes
ab242044 has not performed satisfactorily when used for WB of Epsin 2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Epsin 2.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to Epsin 2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab242044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Plays a role in the formation of clathrin-coated invaginations and endocytosis. -
Tissue specificity
Highest expression is found in brain. Detected at lower levels in lung and liver. -
Sequence similarities
Belongs to the epsin family.
Contains 1 ENTH (epsin N-terminal homology) domain.
Contains 2 UIM (ubiquitin-interacting motif) repeats. -
Domain
The NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP-2 and clathrin binding. -
Post-translational
modificationsUbiquitinated. -
Cellular localization
Cytoplasm. Cytoplasmic vesicle > clathrin-coated vesicle. In punctate structures throughout the cell, associated with clathrin-coated vesicles, and particularly concentrated in the region of the Golgi complex. - Information by UniProt
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Database links
- Entrez Gene: 22905 Human
- Entrez Gene: 13855 Mouse
- Omim: 607263 Human
- SwissProt: O95208 Human
- SwissProt: Q8CHU3 Mouse
- Unigene: 309467 Human
- Unigene: 139695 Mouse
- Unigene: 436466 Mouse
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Alternative names
- EHB21 antibody
- EPN 2 antibody
- Epn2 antibody
see all
Images
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Immunoprecipitation - Anti-Epsin 2 antibody (ab242044)
Epsin 2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab242044 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab242044 at 0.4 µg/ml.
Lane 1: ab242044 IP in HeLa whole cell lysate.
lane 2: Control IgG in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 30 seconds.
Prepared using NETN lysis buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab242044 has not yet been referenced specifically in any publications.
