Product nameAnti-EPSTI1 antibody
See all EPSTI1 primary antibodies
DescriptionRabbit polyclonal to EPSTI1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- IHC: Human duodenum tissue. WB: RT4 and U-251 MG sp cells; human liver tissue lysate. ICC/IF: A431 cells.
This product was previously labelled as Epithelial Stromal Interaction 1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/250. Predicted molecular weight: 37 kDa.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
Tissue specificityHighly expressed in placenta, small intestine, spleen, kidney, thymus, liver, salivary gland and testes. Weakly expressed in breast, skeletal muscle and colon. Highly expressed in breast cancer upon interaction between tumor cells and stromal cells in vitro. Expressed in blood mononuclear cells from patients with systemic lupus erythematosus (SLE).
- Information by UniProt
- BRESI 1 antibody
- BRESI1 antibody
- Epithelial stromal interaction 1 (breast) antibody
All lanes : Anti-EPSTI1 antibody (ab224397)
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG sp (human brain glioma cell line) cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue lysate
Predicted band size: 37 kDa
Paraffin-embedded human duodenum tissue stained for EPSTI1 using ab224397 at 1/20 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for EPSTI1 (green) using ab224397 at 4 µg/ml in ICC/IF.
ab224397 has not yet been referenced specifically in any publications.