The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 81 kDa).
Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis.
Involvement in disease
Defects in EPX are the cause of eosinophil peroxidase deficiency (EPD) [MIM:261500]. EPD is an autosomal recessive defect where anomalous eosinophils are characterized by nuclear hypersegmentation, hypogranulation, and negative peroxidase and phospholipid staining.
Belongs to the peroxidase family. XPO subfamily.
Cytoplasmic granule. Cytoplasmic granules of eosinophils.
We hypothesise that this antibody is detecting the EPX monomer (~70kDa) and the cleaved heavy chain(55kDa). Abcam welcomes feedback from customers working on this protein and would appreciate any comments regarding this product and the data presented above.