Key features and details
- Rabbit polyclonal to EPX
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EPX antibody
See all EPX primary antibodies
DescriptionRabbit polyclonal to EPX
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human EPX aa 400-500 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following human tissue lysates: Bone Marrow; Thymus; Spleen.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab65319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 81 kDa).|
FunctionMediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis.
Involvement in diseaseDefects in EPX are the cause of eosinophil peroxidase deficiency (EPD) [MIM:261500]. EPD is an autosomal recessive defect where anomalous eosinophils are characterized by nuclear hypersegmentation, hypogranulation, and negative peroxidase and phospholipid staining.
Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
Cellular localizationCytoplasmic granule. Cytoplasmic granules of eosinophils.
- Information by UniProt
- Eosinophil peroxidase [Precursor] antibody
- Eosinophil peroxidase antibody
- Eosinophil peroxidase heavy chain antibody
All lanes : Anti-EPX antibody (ab65319) at 1 µg/ml
Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 2 : Human thymus tissue lysate - total protein (ab30146)
Lane 3 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 55 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
We hypothesise that this antibody is detecting the EPX monomer (~70kDa) and the cleaved heavy chain(55kDa). Abcam welcomes feedback from customers working on this protein and would appreciate any comments regarding this product and the data presented above.
ab65319 has not yet been referenced specifically in any publications.