Overview

  • Product name

  • Description

    Rabbit polyclonal to EPX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human EPX aa 400-500 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab104963)

  • Positive control

    • This antibody gave a positive signal in the following human tissue lysates: Bone Marrow; Thymus; Spleen.

Properties

Applications

Our Abpromise guarantee covers the use of ab65319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 81 kDa).

Target

  • Function

    Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis.
  • Involvement in disease

    Defects in EPX are the cause of eosinophil peroxidase deficiency (EPD) [MIM:261500]. EPD is an autosomal recessive defect where anomalous eosinophils are characterized by nuclear hypersegmentation, hypogranulation, and negative peroxidase and phospholipid staining.
  • Sequence similarities

    Belongs to the peroxidase family. XPO subfamily.
  • Cellular localization

    Cytoplasmic granule. Cytoplasmic granules of eosinophils.
  • Information by UniProt
  • Database links

  • Alternative names

    • Eosinophil peroxidase [Precursor] antibody
    • Eosinophil peroxidase antibody
    • Eosinophil peroxidase heavy chain antibody
    • EPER antibody
    • EPO antibody
    • EPP antibody
    • EPX antibody
    • EPX PEN antibody
    • PERE antibody
    • PERE_HUMAN antibody
    see all

Images

  • All lanes : Anti-EPX antibody (ab65319) at 1 µg/ml

    Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Human thymus tissue lysate - total protein (ab30146)
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 81 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 15 kDa, 55 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    We hypothesise that this antibody is detecting the EPX monomer (~70kDa) and the cleaved heavy chain(55kDa). Abcam welcomes feedback from customers working on this protein and would appreciate any comments regarding this product and the data presented above.

References

ab65319 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab65319.
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