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    er-staining-kit-green-fluorescence-cytopainter-ab139481.pdf

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Kits/ Lysates/ Other Kits Cell Staining Kits Endoplasmic Reticulum Staining Kits
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ER Staining Kit - Green Fluorescence - Cytopainter (ab139481)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1) Submit a question References (5)

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Fluorescent staining using CytoPainter ER Staining kit
  • Absorbance and emission spectra for CytoPainter ER Staining kit

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Overview

  • Product name

    ER Staining Kit - Green Fluorescence - Cytopainter
    See all Endoplasmic reticulum kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    ER Staining Kit - Green Fluorescence | Cytopainter (ab139481) contains an endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining. Micromolar concentrations of the green dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line Jurkat and human bone osteosarcoma epithelial cell line, U2OS. One important application of ab139481 is in fluorescence co-localization imaging with red fluorescent protein (RFP)- or orange fluorescent protein (OFP)-tagged proteins, a powerful approach for determining the targeting of molecules to intracellular compartments and for screening of their associations and interactions.


    ab139481 is specifically designed for use with RFP- or OFP-expressing cell lines, as well as cells expressing blue or cyan fluorescent proteins (BFPs, CFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as Texas Red, or coumarin. A nuclear counterstain is provided to highlight this organelle as well.


    Review other dyes and kits for ER staining, or the live cell staining fluorescent dyes guide

  • Notes

    This kit can be readily used in combination with other common UV and visible light excitable organic fluorescent dyes and various fluorescent proteins in multi-color imaging and detection applications. The dye emits in the FITC region of the visible light spectrum, and is resistant to photo-bleaching, concentration quenching and photoconversion.

  • Platform

    Fluorescence microscope

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 500 tests
    10X Assay Buffer 1 x 15ml
    Green Detection Reagent 1 x 50µl
    Hoechst 33342 Nuclear Stain 1 x 50µl
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Cell Staining Kits
    • Endoplasmic Reticulum Staining Kits
  • Alternative names

    • ER

Images

  • Fluorescent staining using CytoPainter ER Staining kit
    Fluorescent staining using CytoPainter ER Staining kit
    Green Detection Reagent staining co-localizes with calreticulin protein containing the KDEL sequence, fused to orange fluorescent protein (OFP).
  • Absorbance and emission spectra for CytoPainter ER Staining kit
    Absorbance and emission spectra for CytoPainter ER Staining kit
    Absorbance and emission spectra for ab139481 (panel A) and Hoechst 33342 dye (panel B). All spectra were determined in 1X Assay Buffer.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab139481? Please let us know so that we can cite the reference in this datasheet.

ab139481 has been referenced in 5 publications.

  • Tuescher JM  et al. Extracts Prepared from a Canadian Toxic Plant Induce Light-Dependent Perinuclear Vacuoles in Human Cells. Toxins (Basel) 13:N/A (2021). PubMed: 33673235
  • Zhi Q  et al. Podocalyxin-like protein promotes gastric cancer progression through interacting with RUN and FYVE domain containing 1 protein. Cancer Sci 110:118-134 (2019). PubMed: 30407695
  • Trigo D  et al. The regulation of mitochondrial dynamics in neurite outgrowth by retinoic acid receptor ß signaling. FASEB J 33:7225-7235 (2019). PubMed: 30857414
  • Kim KS  et al. Oral Nanoparticles Exhibit Specific High-Efficiency Intestinal Uptake and Lymphatic Transport. ACS Nano 12:8893-8900 (2018). PubMed: 30088412
  • Aasen SN  et al. A Novel Nanoprobe for Multimodal Imaging Is Effectively Incorporated into Human Melanoma Metastatic Cell Lines. Int J Mol Sci 16:21658-80 (2015). PubMed: 26370983

Customer reviews and Q&As

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CytoPainter ER staining in mouse primary culture

Poor Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
I tried to stain primary culture derived from mouse cortex, but I received no signal at all.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Bella Koltun

Verified customer

Submitted Jul 04 2022

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