Product nameER Staining Kit - Green Fluorescence - Cytopainter
See all Endoplasmic reticulum kits
Sample typeAdherent cells, Suspension cells
Species reactivityReacts with: Mammals, Other species
ER Staining Kit - Green Fluorescence | Cytopainter (ab139481) contains an endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining. Micromolar concentrations of the green dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line Jurkat and human bone osteosarcoma epithelial cell line, U2OS. One important application of ab139481 is in fluorescence co-localization imaging with red fluorescent protein (RFP)- or orange fluorescent protein (OFP)-tagged proteins, a powerful approach for determining the targeting of molecules to intracellular compartments and for screening of their associations and interactions.
ab139481 is specifically designed for use with RFP- or OFP-expressing cell lines, as well as cells expressing blue or cyan fluorescent proteins (BFPs, CFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as Texas Red, or coumarin. A nuclear counterstain is provided to highlight this organelle as well.
This kit can be readily used in combination with other common UV and visible light excitable organic fluorescent dyes and various fluorescent proteins in multi-color imaging and detection applications. The dye emits in the FITC region of the visible light spectrum, and is resistant to photo-bleaching, concentration quenching and photoconversion.
Storage instructionsPlease refer to protocols.
Components 500 tests 10X Assay Buffer 1 x 15ml Green Detection Reagent 1 x 50µl Hoechst 33342 Nuclear Stain 1 x 50µl
This product has been referenced in:
- Zhi Q et al. Podocalyxin-like protein promotes gastric cancer progression through interacting with RUN and FYVE domain containing 1 protein. Cancer Sci 110:118-134 (2019). Read more (PubMed: 30407695) »
- Kim KS et al. Oral Nanoparticles Exhibit Specific High-Efficiency Intestinal Uptake and Lymphatic Transport. ACS Nano 12:8893-8900 (2018). Read more (PubMed: 30088412) »