ER Staining Kit - Green Fluorescence - Cytopainter (ab139481)
Overview
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Product name
ER Staining Kit - Green Fluorescence - Cytopainter
See all Endoplasmic reticulum kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
ER Staining Kit - Green Fluorescence | Cytopainter (ab139481) contains an endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining. Micromolar concentrations of the green dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line Jurkat and human bone osteosarcoma epithelial cell line, U2OS. One important application of ab139481 is in fluorescence co-localization imaging with red fluorescent protein (RFP)- or orange fluorescent protein (OFP)-tagged proteins, a powerful approach for determining the targeting of molecules to intracellular compartments and for screening of their associations and interactions.
ab139481 is specifically designed for use with RFP- or OFP-expressing cell lines, as well as cells expressing blue or cyan fluorescent proteins (BFPs, CFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as Texas Red, or coumarin. A nuclear counterstain is provided to highlight this organelle as well.
Review other dyes and kits for ER staining, or the live cell staining fluorescent dyes guide
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Notes
This kit can be readily used in combination with other common UV and visible light excitable organic fluorescent dyes and various fluorescent proteins in multi-color imaging and detection applications. The dye emits in the FITC region of the visible light spectrum, and is resistant to photo-bleaching, concentration quenching and photoconversion.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Please refer to protocols. -
Components 500 tests 10X Assay Buffer 1 x 15ml Green Detection Reagent 1 x 50µl Hoechst 33342 Nuclear Stain 1 x 50µl -
Research areas
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Alternative names
- ER
Images
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Fluorescent staining using CytoPainter ER Staining kitGreen Detection Reagent staining co-localizes with calreticulin protein containing the KDEL sequence, fused to orange fluorescent protein (OFP).
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Absorbance and emission spectra for CytoPainter ER Staining kitAbsorbance and emission spectra for ab139481 (panel A) and Hoechst 33342 dye (panel B). All spectra were determined in 1X Assay Buffer.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab139481 has been referenced in 5 publications.
- Tuescher JM et al. Extracts Prepared from a Canadian Toxic Plant Induce Light-Dependent Perinuclear Vacuoles in Human Cells. Toxins (Basel) 13:N/A (2021). PubMed: 33673235
- Zhi Q et al. Podocalyxin-like protein promotes gastric cancer progression through interacting with RUN and FYVE domain containing 1 protein. Cancer Sci 110:118-134 (2019). PubMed: 30407695
- Trigo D et al. The regulation of mitochondrial dynamics in neurite outgrowth by retinoic acid receptor ß signaling. FASEB J 33:7225-7235 (2019). PubMed: 30857414
- Kim KS et al. Oral Nanoparticles Exhibit Specific High-Efficiency Intestinal Uptake and Lymphatic Transport. ACS Nano 12:8893-8900 (2018). PubMed: 30088412
- Aasen SN et al. A Novel Nanoprobe for Multimodal Imaging Is Effectively Incorporated into Human Melanoma Metastatic Cell Lines. Int J Mol Sci 16:21658-80 (2015). PubMed: 26370983