Overview

  • Product name

    ER Staining Kit - Green Fluorescence - Cytopainter
    See all Endoplasmic reticulum kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    ER Staining Kit - Green Fluorescence | Cytopainter (ab139481) contains an endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining. Micromolar concentrations of the green dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line Jurkat and human bone osteosarcoma epithelial cell line, U2OS. One important application of ab139481 is in fluorescence co-localization imaging with red fluorescent protein (RFP)- or orange fluorescent protein (OFP)-tagged proteins, a powerful approach for determining the targeting of molecules to intracellular compartments and for screening of their associations and interactions.


    ab139481 is specifically designed for use with RFP- or OFP-expressing cell lines, as well as cells expressing blue or cyan fluorescent proteins (BFPs, CFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as Texas Red, or coumarin. A nuclear counterstain is provided to highlight this organelle as well.


    Review other dyes and kits for ER staining, or the live cell staining fluorescent dyes guide

  • Notes

    This kit can be readily used in combination with other common UV and visible light excitable organic fluorescent dyes and various fluorescent proteins in multi-color imaging and detection applications. The dye emits in the FITC region of the visible light spectrum, and is resistant to photo-bleaching, concentration quenching and photoconversion.

  • Platform

    Fluorescence microscope

Properties

Images

  • Green Detection Reagent staining co-localizes with calreticulin protein containing the KDEL sequence, fused to orange fluorescent protein (OFP).
  • Absorbance and emission spectra for ab139481 (panel A) and Hoechst 33342 dye (panel B). All spectra were determined in 1X Assay Buffer.

Protocols

References

This product has been referenced in:

  • Zhi Q  et al. Podocalyxin-like protein promotes gastric cancer progression through interacting with RUN and FYVE domain containing 1 protein. Cancer Sci 110:118-134 (2019). Read more (PubMed: 30407695) »
  • Kim KS  et al. Oral Nanoparticles Exhibit Specific High-Efficiency Intestinal Uptake and Lymphatic Transport. ACS Nano 12:8893-8900 (2018). Read more (PubMed: 30088412) »
See all 3 Publications for this product

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