Product nameER Staining Kit - Red Fluorescence - Cytopainter
See all Endoplasmic reticulum kits
Sample typeAdherent cells, Suspension cells
Species reactivityReacts with: Mammals, Other species
ER Staining Kit - Red Fluorescence | Cytopainter (ab139482) contains an endoplasmic reticulum-selective red dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining. Micromolar concentrations of the red dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line Jurkat and human bone osteosarcoma epithelial cell line, U2OS.
One important application of the ER staining kit is in fluorescence co-localization imaging with green fluorescent protein (GFP)-tagged proteins, a powerful approach for determining the targeting of molecules to intracellular compartments and for screening of their associations and interactions.
The ER staining kit is specifically designed for use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFPs, CFPs, YFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as fluorescein, or coumarin.
A nuclear counterstain is also included in the kit.
This kit can be readily used in combination with other common UV and visible light excitable organic fluorescent dyes and various fluorescent proteins in multi-color imaging and detection applications. The dye emits in the Texas Red region of the visible light spectrum, and is resistant to photo-bleaching, concentration quenching and photoconversion.
Previously called CytoPainter ER Staining Kit -Red Fluorescence.
Live HeLa cells stained with Red Detection Reagent (A), Hoechst dye (B) and resulting composite image (C).
Fluorescence excitation and emission spectra for Red dye (panel A) and absorbance and fluorescent emission spectra for Hoechst 33342 dye (panel B). All spectra were determined in 1X Assay Buffer.
ab139482 has been referenced in 4 publications.
- Khan ES et al. Exogenous supply of Hsp47 triggers fibrillar collagen deposition in skin cell cultures in vitro. BMC Mol Cell Biol 21:22 (2020). PubMed: 32228452
- Mao F et al. Hemocyte phagosomal proteome is dynamically shaped by cytoskeleton remodeling and interorganellar communication with endoplasmic reticulum during phagocytosis in a marine invertebrate, Crassostrea gigas. Sci Rep 10:6577 (2020). PubMed: 32313134
- Bilska A et al. Immunoglobulin expression and the humoral immune response is regulated by the non-canonical poly(A) polymerase TENT5C. Nat Commun 11:2032 (2020). PubMed: 32341344
- Liu T et al. Glycosylation controls sodium-calcium exchanger 3 sub-cellular localization during cell cycle. Eur J Cell Biol 97:190-203 (2018). PubMed: 29526322