ER Staining Kit - Red Fluorescence - Cytopainter (ab139482)
Overview
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Product name
ER Staining Kit - Red Fluorescence - Cytopainter
See all Endoplasmic reticulum kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
ER Staining Kit - Red Fluorescence | Cytopainter (ab139482) contains an endoplasmic reticulum-selective red dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining. Micromolar concentrations of the red dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line Jurkat and human bone osteosarcoma epithelial cell line, U2OS. (Ex/Em = 580/677 nm).
One important application of the ER staining kit is in fluorescence co-localization imaging with green fluorescent protein (GFP)-tagged proteins, a powerful approach for determining the targeting of molecules to intracellular compartments and for screening of their associations and interactions.
The ER staining kit is specifically designed for use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFPs, CFPs, YFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as fluorescein, or coumarin.
A nuclear counterstain is also included in the kit.
Review other dyes and kits for ER staining, or the live cell staining fluorescent dyes guide
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Notes
This kit can be readily used in combination with other common UV and visible light excitable organic fluorescent dyes and various fluorescent proteins in multi-color imaging and detection applications. The dye emits in the Texas Red region of the visible light spectrum, and is resistant to photo-bleaching, concentration quenching and photoconversion.
Previously called CytoPainter ER Staining Kit -Red Fluorescence.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Please refer to protocols. -
Components 500 tests 10X Assay Buffer 1 x 15ml Hoechst 33342 Nuclear Stain 1 x 50µl Red Detection Reagent 1 x 50µl -
Research areas
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Alternative names
- ER
Images
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Fluorescent staining using CytoPainter ER Staining kitLive HeLa cells stained with Red Detection Reagent (A), Hoechst dye (B) and resulting composite image (C).
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Fluorescence excitation and emission spectra for CytoPainter ER Staining kitFluorescence excitation and emission spectra for Red dye (panel A) and absorbance and fluorescent emission spectra for Hoechst 33342 dye (panel B). All spectra were determined in 1X Assay Buffer.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab139482 has been referenced in 8 publications.
- Jang H et al. FCN3 functions as a tumor suppressor of lung adenocarcinoma through induction of endoplasmic reticulum stress. Cell Death Dis 12:407 (2021). PubMed: 33859174
- Feng D et al. Regulation of Wnt/PCP signaling through p97/VCP-KBTBD7-mediated Vangl ubiquitination and endoplasmic reticulum-associated degradation. Sci Adv 7:N/A (2021). PubMed: 33990333
- Khan ES et al. Exogenous supply of Hsp47 triggers fibrillar collagen deposition in skin cell cultures in vitro. BMC Mol Cell Biol 21:22 (2020). PubMed: 32228452
- Mao F et al. Hemocyte phagosomal proteome is dynamically shaped by cytoskeleton remodeling and interorganellar communication with endoplasmic reticulum during phagocytosis in a marine invertebrate, Crassostrea gigas. Sci Rep 10:6577 (2020). PubMed: 32313134
- Bilska A et al. Immunoglobulin expression and the humoral immune response is regulated by the non-canonical poly(A) polymerase TENT5C. Nat Commun 11:2032 (2020). PubMed: 32341344
- Garufi A et al. Interplay between Endoplasmic Reticulum (ER) Stress and Autophagy Induces Mutant p53H273 Degradation. Biomolecules 10:N/A (2020). PubMed: 32138264
- Janezic EM et al. N-glycosylation of a1D-adrenergic receptor N-terminal domain is required for correct trafficking, function, and biogenesis. Sci Rep 10:7209 (2020). PubMed: 32350295
- Liu T et al. Glycosylation controls sodium-calcium exchanger 3 sub-cellular localization during cell cycle. Eur J Cell Biol 97:190-203 (2018). PubMed: 29526322