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ER Staining Kit - Red Fluorescence - Cytopainter (ab139482)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (4)Q&A (1)References (4)

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Fluorescent staining using CytoPainter ER Staining kit
  • Fluorescence excitation and emission spectra for CytoPainter ER Staining kit

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Overview

  • Product name

    ER Staining Kit - Red Fluorescence - Cytopainter
    See all Endoplasmic reticulum kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    ER Staining Kit - Red Fluorescence | Cytopainter (ab139482) contains an endoplasmic reticulum-selective red dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining. Micromolar concentrations of the red dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line Jurkat and human bone osteosarcoma epithelial cell line, U2OS.


    One important application of the ER staining kit is in fluorescence co-localization imaging with green fluorescent protein (GFP)-tagged proteins, a powerful approach for determining the targeting of molecules to intracellular compartments and for screening of their associations and interactions.


    The ER staining kit is specifically designed for use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFPs, CFPs, YFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as fluorescein, or coumarin.


    A nuclear counterstain is also included in the kit.


    Review other dyes and kits for ER staining, or the live cell staining fluorescent dyes guide

  • Notes

    This kit can be readily used in combination with other common UV and visible light excitable organic fluorescent dyes and various fluorescent proteins in multi-color imaging and detection applications. The dye emits in the Texas Red region of the visible light spectrum, and is resistant to photo-bleaching, concentration quenching and photoconversion.

    Previously called CytoPainter ER Staining Kit -Red Fluorescence.

  • Platform

    Fluorescence microscope

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 500 tests
    10X Assay Buffer 1 x 15ml
    Hoechst 33342 Nuclear Stain 1 x 50µl
    Red Detection Reagent 1 x 50µl
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Cell Staining Kits
    • Endoplasmic Reticulum Staining Kits
  • Alternative names

    • ER

Associated products

    Images

    • Fluorescent staining using CytoPainter ER Staining kit
      Fluorescent staining using CytoPainter ER Staining kit
      Live HeLa cells stained with Red Detection Reagent (A), Hoechst dye (B) and resulting composite image (C).
    • Fluorescence excitation and emission spectra for CytoPainter ER Staining kit
      Fluorescence excitation and emission spectra for CytoPainter ER Staining kit
      Fluorescence excitation and emission spectra for Red dye (panel A) and absorbance and fluorescent emission spectra for Hoechst 33342 dye (panel B). All spectra were determined in 1X Assay Buffer.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (4)

    Publishing research using ab139482? Please let us know so that we can cite the reference in this datasheet.

    ab139482 has been referenced in 4 publications.

    • Khan ES  et al. Exogenous supply of Hsp47 triggers fibrillar collagen deposition in skin cell cultures in vitro. BMC Mol Cell Biol 21:22 (2020). PubMed: 32228452
    • Mao F  et al. Hemocyte phagosomal proteome is dynamically shaped by cytoskeleton remodeling and interorganellar communication with endoplasmic reticulum during phagocytosis in a marine invertebrate, Crassostrea gigas. Sci Rep 10:6577 (2020). PubMed: 32313134
    • Bilska A  et al. Immunoglobulin expression and the humoral immune response is regulated by the non-canonical poly(A) polymerase TENT5C. Nat Commun 11:2032 (2020). PubMed: 32341344
    • Liu T  et al. Glycosylation controls sodium-calcium exchanger 3 sub-cellular localization during cell cycle. Eur J Cell Biol 97:190-203 (2018). PubMed: 29526322

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-4 of 4 Abreviews

    Combining ER dye and vimentin antibody staining

    Good Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    HUVEC (30000) were seeded 8-well ibiTreat ibidi culture slides, and grown until nearly confluent for 24-48h.
    Cells were washed with PBS, fixated with 1% PFA for 20' RT and subsequently permeabilized with PBS/0.1%Triton-X100 for 10’RT.
    Cells were incubated with primary Ab (mouse anti-vimentin, clone V9, Dako, 1:200), secondary goat-anti-mouse biotin (Dako, 1:200) and streptavidin-Alexa488 (ThermoFisher 1:500), all diluted in PBS/0.5%BSA. All incubations were for 45-60' RT in the dark, followed by 3 washes with PBS.
    Cytopainter ER staining dye was diluted 1:1000 in 1x assay buffer, added to the cells, and incubated for 30' at 37C. If applicable, Hoechst was also diluted 1:1000 in the same buffer.
    Cells were washed 2x in PBS before images were taken on a Leica DMIL inverted microscope equipped with a Leica DFC345FX camera, and processed in Leica Application Suite software.

    Figure:
    HUVEC stained with Cytopainter ER staining dye (red) Hoechst (blue) and anti-vimentin Ab (green).

    Judy Van Beijnum

    Verified customer

    Submitted Oct 26 2017

    Chicken Fibroblasts

    Good Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    Chicken Embryonic Fibroblasts (CEF) stained according to Abcam's recommended protocol.

    Dr. Marcelo Pelajo Machado

    Verified customer

    Submitted May 09 2017

    Economic and very easy to use

    Good Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    1. Mouse astrocyte primary culture was fixed in 4%PFA for 5 minutes.
    2. Block and permeabilized o/n, 4C with 1%BSA+0.1% Triton-X.
    3. In 1X buffer: 1:1000 Texas red (ER) and 1:1000 Hoestch, room temperature for 40 minutes,
    4. Rinse with 1X buffer twice and mount.
    Imaged in a Leica SP8, 40X magnification, 1024x1024.

    Ms. Maria Velasco

    Verified customer

    Submitted Mar 17 2017

    ab139482 for mouse tissue

    Good Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    This kit is good!

    Abcam user community

    Verified customer

    Submitted Jun 18 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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