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Product code: 139482
Inquiry: Hi, our group bought the cytopainter ER ab139482 to staining HeLa cells but we found the reagent being very toxic and induces huge cell detachment. We followed the protocol to dilute the reagent (warm up reagent to 37, keep cells at 37, etc), we even tried to further dilute the ER dye but we still haven’t had a good staining.
Asked on May 04 2018
Staining at low concentrations does not appear to be toxic to cells. I know that you already tried to reduce the concentration, maybe you can try to reduce it more.
Customer feedback: I've tested the ER staining on Friday and the 0.2X concentration can stain HeLa cells well without causing too much toxicity. It is good new but we spent quite some effort to obtain the same results in HeLa as you advertise on the website. In contrast, the staining using Mitochondria and lysosome Cytopainter were just as you described. We've used many of your products and this ER stain protocol was the least satisfying one so far. Hope the information could be modified and cost less optimisation time in future.
Answered on May 04 2018