Key features and details
- Mouse monoclonal [5F3] to ERAB
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Product nameAnti-ERAB antibody [5F3]
See all ERAB primary antibodies
DescriptionMouse monoclonal [5F3] to ERAB
SpecificityThis antibody detects the recombinant human ERAB protein (27kDa) and recognizes the endogenous ERAB protein in cell extracts with virtually no crossreactivity with other human proteins.
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Recombinant, full-length Human ERAB protein (fusion protein).
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
Purification notesAmmonium sulfate precipitated and dialyzed tissue culture supernatant.
Light chain typelambda
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/1000.
1/100 - 1/1000.
FunctionFunctions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/RG9MTD1, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD).
Tissue specificityExpressed in normal tissues but is overexpressed in neurons affected in AD.
Involvement in diseaseDefects in HSD17B10 are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD deficiency) [MIM:300438]. MHBD deficiency leads to neurological abnormalities, including psychomotor retardation, and, in virtually all patients, loss of mental and motor skills.
Defects in HSD17B10 are the cause of mental retardation syndromic X-linked type 10 (MRXS10) [MIM:300220]. MRXS10 is characterized by mild mental retardation, choreoathetosis and abnormal behavior.
A chromosomal microduplication involving HSD17B10 and HUWE1 is the cause of mental retardation X-linked type 17 (MRX17) [MIM:300705]; also known as mental retardation X-linked type 31 (MRX31). Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X-linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation.
Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
- Information by UniProt
- 17 beta hydroxysteroid dehydrogenase 10 antibody
- 17 beta hydroxysteroid dehydrogenase type 10 antibody
- 17-beta-HSD 10 antibody
IHC staining of purifiedab10260 on formalin-fixed paraffin-embedded human colon tissue. The tissue was incubated with 10 µg/ml of the primary antibody for 60 minutes at room temperature. A HRP kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
ab10260 has been referenced in 6 publications.
- Liu L et al. Deacetylation of HSD17B10 by SIRT3 regulates cell growth and cell resistance under oxidative and starvation stresses. Cell Death Dis 11:563 (2020). PubMed: 32703935
- Xiao X et al. ABAD/17ß-HSD10 reduction contributes to the protective mechanism of huperzine a on the cerebral mitochondrial function in APP/PS1 mice. Neurobiol Aging 81:77-87 (2019). PubMed: 31252207
- Bertolin G et al. The TOMM machinery is a molecular switch in PINK1 and PARK2/PARKIN-dependent mitochondrial clearance. Autophagy 9:1801-17 (2013). ICC/IF ; Human . PubMed: 24149440
- Kitchens CA et al. Identification of chemosensitivity nodes for vinblastine through small interfering RNA high-throughput screens. J Pharmacol Exp Ther 339:851-8 (2011). Human . PubMed: 21880871
- Onoguchi K et al. Virus-infection or 5'ppp-RNA activates antiviral signal through redistribution of IPS-1 mediated by MFN1. PLoS Pathog 6:e1001012 (2010). WB, ICC/IF ; Human . PubMed: 20661427
- Stevanin G et al. Mutations in SPG11, encoding spatacsin, are a major cause of spastic paraplegia with thin corpus callosum. Nat Genet 39:366-372 (2007). PubMed: 17322883