Overview

  • Product name
    Anti-ERAB antibody [5F3]
    See all ERAB primary antibodies
  • Description
    Mouse monoclonal [5F3] to ERAB
  • Host species
    Mouse
  • Specificity
    This antibody detects the recombinant human ERAB protein (27kDa) and recognizes the endogenous ERAB protein in cell extracts with virtually no crossreactivity with other human proteins.
  • Tested applications
    Suitable for: WB, Dot blot, IHC-P, IHC-Fr, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant, full-length Human ERAB protein (fusion protein).

Properties

Applications

Our Abpromise guarantee covers the use of ab10260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/10000. Predicted molecular weight: 27 kDa.
Dot blot 1/100 - 1/10000.
IHC-P 1/100 - 1/1000.
IHC-Fr 1/100 - 1/1000.
ELISA Use at an assay dependent concentration.
ICC/IF 1/2000. PubMed: 17322883

Target

  • Function
    Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/RG9MTD1, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD).
  • Tissue specificity
    Expressed in normal tissues but is overexpressed in neurons affected in AD.
  • Involvement in disease
    Defects in HSD17B10 are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD deficiency) [MIM:300438]. MHBD deficiency leads to neurological abnormalities, including psychomotor retardation, and, in virtually all patients, loss of mental and motor skills.
    Defects in HSD17B10 are the cause of mental retardation syndromic X-linked type 10 (MRXS10) [MIM:300220]. MRXS10 is characterized by mild mental retardation, choreoathetosis and abnormal behavior.
    A chromosomal microduplication involving HSD17B10 and HUWE1 is the cause of mental retardation X-linked type 17 (MRX17) [MIM:300705]; also known as mental retardation X-linked type 31 (MRX31). Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X-linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation.
  • Sequence similarities
    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 17 beta hydroxysteroid dehydrogenase 10 antibody
    • 17 beta hydroxysteroid dehydrogenase type 10 antibody
    • 17-beta-HSD 10 antibody
    • 17-beta-hydroxysteroid dehydrogenase 10 antibody
    • 17b HSD10 antibody
    • 3 hydroxy 2 methylbutyryl CoA dehydrogenase antibody
    • 3 hydroxyacyl CoA dehydrogenase type 2 antibody
    • 3 hydroxyacyl CoA dehydrogenase type II antibody
    • 3-hydroxy-2-methylbutyryl-CoA dehydrogenase antibody
    • 3-hydroxyacyl-CoA dehydrogenase type II antibody
    • 3-hydroxyacyl-CoA dehydrogenase type-2 antibody
    • AB binding alcohol dehydrogenase antibody
    • ABAD antibody
    • Ads9 antibody
    • Amyloid beta binding polypeptide antibody
    • Amyloid beta peptide binding alcohol dehydrogenase antibody
    • Amyloid beta peptide binding protein antibody
    • Amyloid beta peptide binding protein antibody
    • CAMR antibody
    • DUPXp11.22 antibody
    • Endoplasmic Reticulum Amyloid Binding Protein antibody
    • Endoplasmic reticulum associated amyloid beta peptide binding protein antibody
    • Endoplasmic reticulum-associated amyloid beta-peptide-binding protein antibody
    • ER associated amyloid beta-binding protein antibody
    • ERAB antibody
    • HADH 2 antibody
    • HADH2 antibody
    • HCD 2 antibody
    • HCD2 antibody
    • HCD2_HUMAN antibody
    • Hsd17b10 antibody
    • Hydroxyacyl CoA Dehydrogenase type II antibody
    • Hydroxyacyl Coenzyme A dehydrogenase type II antibody
    • Hydroxysteroid (17 beta) dehydrogenase 10 antibody
    • Mental retardation X linked syndromic 11 antibody
    • MHBD antibody
    • Mitochondrial L3 Hydroxyacyl CoA Dehydrogenase antibody
    • Mitochondrial ribonuclease P protein 2 antibody
    • Mitochondrial RNase P protein 2 antibody
    • MRPP2 antibody
    • MRX17 antibody
    • SCHAD antibody
    • SDR5C1 antibody
    • Short chain dehydrogenase/reductase family 5C member 1 antibody
    • Short chain L 3 hydroxyacyl CoA dehydrogenase type 2 antibody
    • Short chain type dehydrogenase/reductase XH98G2 antibody
    • Short-chain type dehydrogenase/reductase XH98G2 antibody
    • Type 10 17b HSD antibody
    • Type 10 17beta hydroxysteroid dehydrogenase antibody
    • Type II HADH antibody
    • XH98G2 antibody
    see all

Images

  • IF using ab10260.

References

This product has been referenced in:
  • Bertolin G  et al. The TOMM machinery is a molecular switch in PINK1 and PARK2/PARKIN-dependent mitochondrial clearance. Autophagy 9:1801-17 (2013). ICC/IF ; Human . Read more (PubMed: 24149440) »
  • Kitchens CA  et al. Identification of chemosensitivity nodes for vinblastine through small interfering RNA high-throughput screens. J Pharmacol Exp Ther 339:851-8 (2011). Human . Read more (PubMed: 21880871) »
See all 4 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for your email. Unfortunately, we do not know the exact epitope that this product recognizes. We have not done an epitope mapping project for this yet. I apologize for the inconvenience.

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Answer

The application was added to the datasheet based on publication in the following reference:

Stevanin G et al. Mutations in SPG11, encoding spatacsin, are a major cause of spastic paraplegia with thin corpus callosum. Nat Genet 39:366-372 (2007) (PubMed: 17322883).

The antibody was also used in the following reference, for IF (Figure 2) as well as western blotting.

Onoguchi K et al. Virus-infection or 5'ppp-RNA activates antiviral signal through redistribution of IPS-1 mediated by MFN1. PLoS Pathog 6:e1001012 (2010). (PubMed: 20661427).

The protocol in the paper is as follows:

For immunofluorescence analysis, cells were fixed with 4% paraformaldehyde for 10 min, permeabilized with an acetone: methanol (11) solution, and blocked with 5mg/ml of BSA in PBST (PBS, 0.04% Tween20) for 1 hour. The cells were next incubated with relevant primary antibodies overnight at 4°C, and then incubated with relevant Alexa Fluor 405, Alexa Fluor 488, or Alexa Fluor 594-conjugated secondary antibodies.

We are not aware of any data from staining of monkey samples.

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Answer

Thank you for your enquiry. I am sorry that unfortunately, we are unable to supply this anti-ERAB antibody (ab10260) without sodium azide. We do, however, have another anti-ERAB antibody available that contains no preservatives. This is a Mouse monoclonal [10D12] to ERAB, ab17298. Please be aware that non of these antibodies have been tested for your in vivo application. I hope this helps. Please don't hesitate to get back to me if you have any further questions.

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Answer

Thank you for your email. ERAB is identical to SCHAD. The following reference that I found explains this best: Characterization and localization of human type10 17-hydroxysteroid dehydrogenase; Eur. J. Biochem. 268, 4899-4907 (2001). In it the authors state "Human short chain L-3-hydroxyacyl-CoA dehydrogenase (SCHAD) is encoded by the SCHAD gene [10]. Human SCHAD is a member of the SDR family [17] but not the L-3-hydroxyacyl-CoA dehydrogenase (HAD) family [34], because it does not have a signature sequence that defines this family [27,35]. The use of term SCHAD to refer to members of the HAD family has been discouraged because it is inaccurate [36]. It exhibits oxidative 17/3-hydroxysteroid dehydrogenase activities toward sex steroids [11], which leads us to designate this 108-kDa protein as 17-HSD type 10. Because this protein exhibits a high affinity for A peptide and was reported to be associated with the ER, it had been referred to as ER-associated A binding protein (ERAB) [18], and was thought to be a putative A receptor. However, we found that SCHAD is present in mitochondria [11,19]. If this protein is specifically targeted to mitochondria, it seems unlikely that it could readily bind A generated in the ER as had been postulated previously." If you have any additional questions, please contact us again.

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Answer

Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

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Answer

Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused. If you have any more questions, please do contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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